Napredna pretraga

Pregled bibliografske jedinice broj: 394129

Functionally Active Toll-Like Receptor 3 on Human Primary and Metastatic Cancer Cells


Matijević, Tanja; Marjanović, Marko; Pavelić, Jasminka
Functionally Active Toll-Like Receptor 3 on Human Primary and Metastatic Cancer Cells // Scandinavian Journal of Immunology, 70 (2009), 1; 18-24 doi:10.1111/j.1365-3083.2009.02262.x (međunarodna recenzija, članak, znanstveni)


Naslov
Functionally Active Toll-Like Receptor 3 on Human Primary and Metastatic Cancer Cells

Autori
Matijević, Tanja ; Marjanović, Marko ; Pavelić, Jasminka

Izvornik
Scandinavian Journal of Immunology (0300-9475) 70 (2009), 1; 18-24

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Toll-like receptor 3 ; primary and metastatic cancer cells

Sažetak
Toll-like receptor 3 (TLR3) is a member of Toll-like receptors who recognize structurally conserved molecules derived from pathogens and trigger the immune response. To clarify if TLR3, is expressed in certain tumour cell lines and whether it is functional and what is the response of these cell lines to different concentrations of poly I: C treatment, we have screened SW480, SW620, FaDu and Detroit 562 cell lines using real-time PCR, flow cytometry and ELISA. We have shown that all these cell lines express TLR3 on mRNA and protein level but it is only functional in Detroit 562 cell line since only in these cells poly I: C treatment triggered the IL-6 secretion. In addition, poly I: C treatment inhibited cell growth and triggered up-regulation of IL-12p40, IL-8 and Il-1 alpha in Detroit 562 cell line. By using annexin-V apoptosis detection kit, we have found that poly I:C triggers apoptosis in Detroit 562 cell line. We have found here that based on the results of TLR3 functionality there is a huge difference between FaDu and Detroit 562 cell lines which are of the same origin (pharynx) but FaDu is primary and Detroit 562 metastatic carcinoma. Our study also shows that Detroit 562 cell line could be a good model for cancer therapy research and development as it is responsive to TLR3 agonists which consequently drives it to apoptosis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
098-0982464-2394 - Gensko liječenje tumora djelovanjem na molekule imunološkog sustava (Jasminka Pavelić, )
098-0982464-2514 - Uloga različitih mehanizama odgovora stanica na terapiju oštećenjem DNA (Marijeta Kralj, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati