engleski

Genetic requirements for hyper-recombination by very short patch mismatch repair-involvement of Escherichia coli DNA polineras

It has been established that very short patch (VSP) mismatch repair, depending inEscherichia coli on MutL, MutS and Dcm functions, is responsible for the hyper-recombinogenic effect of a class of genetic markers. We show that VSP repair requires the presence of the complete DNA polymerase I enzyme. The absence of endonuclease activities involved in the repair of base-loss sites, Nth, Nfo and Xth, does not affect VSP repair. Implications for the mechanism of the VSP repair are discussed.

escherichia coli ; bacteriophage λ ; DNA polymerase ; AP-endonuclease ; gene conversion

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