engleski

Nm23-H1 overexpressing CAL 27 carcinoma cells exhibit reduced migratory and invasive potential

nm23-H1 was found to diminish metastatic potential of certain carcinoma cell lines and therefore, it was placed in the group of metastatic suppressor genes. Its protein product is one of the two subunits of a well known enzyme - nucleosid diphosphate kinase (NDPK) as well as a protein kinase and nuclease. Since the process of metastasis is dependent on adhesion properties of cells, the goal of our work was to describe the adhesion properties of CAL 27 cells (oral squamous cell carcinoma of the tongue) overexpressing FLAG/nm23-H1 as well as their migratory and invasive potential. The experimental procedures involved standard adhesion tests on various substrata as well as migration and invasion test in Boyden chambers followed by fluorescent immunocytochemistry. In our experiments, we have discovered that cells overexpressing Nm23-H1 adhere faster on substrata (collagen IV and fibronectin) and show more spread morphology than the control cells, while their migratory and invasive potential was reduced. In addition, our results imply that Nm23-H1 is involved in membrane ruffling during early spreading/adhesion and migration. These data suggest an important role of Nm23-H1 in adhesion and migration processes in oral squamous cell carcinoma which contributes to the overall knowledge on nm23 as a metastasis suppressor gene.

CAL 27; adhesion; invasion; motility; nm23

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