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Validation of ICP-OES method for wine macro and microelements determination

Knowledge of macro and microelements content in wine is of outmost interest because of the needs of the wine industry as well as for toxicological implications. Wine macro and micro elements can be determined by different analytical methods from simple gravimetric and volumetric up to sophisticated instrumental methods. Inductively Coupled Plasma Optical Emission Spectrometry is taking more and more distinctive place in the scope of elements determination in broad array of matrixes. The advantages of this method are precision, accuracy, low detection limits and short time for determination of large number of elements with minimum of sample preparation. Validation of ICP-OES method for determination of 16 elements in wine (Al, As, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, Sn, Zn, Ca, K, Mg, Na) was performed. To demonstrate that analytical method of choice is suitable for its intended purpose, appropriate experimental work was designed to provide validation parameters: selectivity, accuracy, precision, linearity and quantification limit. The results obtained showed that method is selective and accurate. 98% average recovery indicates agreement between reference and obtained values. Evaluated data showed high level of precision (1.0, 2.1 and 6.1 % average RSD for repeatability of measurement, standard solution preparation and sample preparation, respectively). Quantification limits were in the level of 0.001-0.005 mg/L for micro and up to 0.2 mg/L for macro elements. The correlation coefficients (r2 at least 0.999), y-intercept, slope of the regression line and residual sum of square were calculated, demonstrating the linearity of the method.

validation; ICP-OES; macro and micro elements; wine

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