engleski

Lipids of the adipose human tissue

Examined were the fat of the kidney capsule and the subcutaneous tissue of 91 patients of different age ( 50-80 years ) and sex. Fat was taken from the autopsy and the surgical operations. The chopped and homogenized adipose tissue was treated with a mixture of chloroform: methanol (2:1) according to Folch. The mild alcalic hydrolysis of the polar lipids isolated the sphingolipids. Different groups of lipids were identified by a thin layer chromatography on the silicagel G with chlorophorm – methanol - water (65:25:4), and phospholipids with chloroform-metanol-7M-ammonia (46:18:3). Adipose human tissue contains 66%-96% of lipids. The main components are nonpolar lipids (95%-99% of the total lipids) and consist of triglycerides and cholesterol. Only 1% of the total lipids are polar: lisolecithin, lisokephalin, lecithin, sulphatide, kephalin, cerebroside, free fat acids and the remaining cholesterol. The slightly increased value for the subcutaneous and kidney fats in women are not significant. The chromatograms on the thin layer silicagel G confirm that there is no difference with regard to sex and the kind of fat in adipose man taken from patients during surgery.

Lipids; Adipose tissue; Human

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