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Pregled bibliografske jedinice broj: 389427

The activity of extracellular signal-regulated kinase (ERK) is required during G2/M phase before metaphase-anaphase transition in synchronized leukemia cell lines


Matković, Katarina; Lukinović-Škudar, Vesna; Banfić, Hrvoje; Višnjić, Dora
The activity of extracellular signal-regulated kinase (ERK) is required during G2/M phase before metaphase-anaphase transition in synchronized leukemia cell lines // International Journal of Hematology, 89 (2009), 2; 159-166 (međunarodna recenzija, članak, znanstveni)


Naslov
The activity of extracellular signal-regulated kinase (ERK) is required during G2/M phase before metaphase-anaphase transition in synchronized leukemia cell lines

Autori
Matković, Katarina ; Lukinović-Škudar, Vesna ; Banfić, Hrvoje ; Višnjić, Dora

Izvornik
International Journal of Hematology (0925-5710) 89 (2009), 2; 159-166

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
ERK; HL-60; NB4; Cell cycle; Aphidicolin; Nocodazole; G2/M

Sažetak
The pharmacological inhibitors of extracellular signal-regulated kinase (ERK) have been suggested as a novel molecular target-based therapy for acute myeloid leukemia. Several studies have established the role of ERK in cell cycle progression from G1 to S phase in response to mitogen, but the role of ERK after the restriction point is less clarified. In this study, we used models of aphidicolin and nocodazole-synchronized HL-60 and NB4 leukemia cell lines to determine the kinetics of ERK-activity during the progression of the cell cycle and to test the effects of commercially available inhibitors on G2/M progression of synchronized leukemia cells. In aphidicolin-synchronized cells, the activity of ERK was low during early S phase and increased at late S and G2/M phase of the cell cycle. The presence of MEK inhibitors PD 98059 and U0126 caused a delay in G2/M phase. In nocodazole-synchronized cells, the activity of ERK was low during M/G1 transition and MEK inhibitors had no effects on return of the cells to G1 phase. These results demonstrate that the activity of ERK is required during G2/M phase of leukemia cell cycle before the cells reach metaphase-anaphase transition.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
108-1081347-0173 - Funkcija fosfoinozitol 3-kinaze C2 beta u staničnim jezgrama (Hrvoje Banfić, )
108-1081347-1448 - Uloga PLC i Akt u staničnom ciklusu i diferencijaciji leukemija (Dora Višnjić, )

Ustanove
Medicinski fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • MEDLINE