engleski

Immunohistochemical analysis of p53 protein in skin tumors

In recent years, the importance of suppressor’ s genes in the manifestation and development of tumors is evident. The aim of the study was to investigate the expression of p35 protein which control the G1-S cell cycle checkpoint, and can trigger apoptosis, in different cutaneous tumors. Tumor samples were obtained from 15 patients with melanoma, 8 patients with naevi, 12 with basal cell carcinoma, 10 with squamous cell carcinoma, and 5 patients with actinic keratoses. Specimens were formalin-fixed, embedded in paraffin wax. 5 μ m sections from the specimen were monted on glass slides, air-dried, dewaxed and rehydrated. After this the specimen were incubated with diluted anti p53 protein monoclonal antibody. Finally, avidin-biotin-peroxidase complex (DAKO-kit) was used. In 41.6% of basal cell carcinoma there were an overexpression of p35 protein in their cell nuclei. In squamous cell cancer we found in 70% expression of p53 protein. In the cases of actinic keratoses the percentage was 20%. In melanoma 70% of the tumors expressed p53 protein and this correlated with its thickness, while in naevi there were not expression of p35 in their cells. The results were compared with the sites (sunlight exposure) of the tumors. In conclusion, these results indicate that there is a negative or slight expression of p53 protein in benign lesion. In contrast, the expression of p53 protein in malignant lesions is significantly increased. The study of p53 in tumors, especially its mutation can contribute to the diagnosis of their etiology, understanding the pathogenesis of the tumors, as well as new insight in their prognosis and therapy.

Immunohistochemical analysis; p53 protein; skin tumors

nije evidentirano