engleski

Umbilical Serum Tumor Necrosis Factor-α and Interleukin-6 in Chorioamnionitis

The term chorioamnionitis describes both clinical symptoms of intrauterine infection and antenatal inflammatory processes in the placental tissue. We studied umbilical serum tumor necrosis factor- α (TNF-α ) and interleukin-6 (IL-6) content in clinical chorioamnionitis with/without histological inflammation and in histological chorioamnionitis without clinical signs. We examined amniotic and placental tissues and umbilical cord blood samples (111) from 40 patients with clinical chorioamnionitis and 71 without it.We stratified patients according to clinical and histological findings. Umbilical serum concentrations of TNF-α and IL-6 were determined with immunoassay. To estimate systemic inflammation in infants, we used cut off values >15 pg/mL for TNF-a and >11 pg/mL for IL-6. To analyze the results, we applied methods of nonparametric statistics. In clinical chorioamnionitis, median umbilical serum concentrations of TNF-α and IL-6 were elevated only when histological inflammation (all P<0.001) was present. In histological chorioamnionitis without clinical signs, median concentrations of TNF- α and IL-6 were also elevated (P<0.001, <0.048). The percentage of elevated cytokines was higher in chorioamnionitis with than without placental inflammation. Cytokine patterns were different in chorioamnionitis patients and controls. Associated clinical and histological chorioamnionitis and histological chorioamnionitis enhanced inflammatory cytokine concentrations and the percentage of elevated cytokine values. The contribution of clinical chorioamnionitis without placental inflammation to umbilical serum cytokine content was minor.

umbilical serum; TNF-α; IL-6; chorioamnionitis

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