engleski

Karyological analysis of three Astacidae species : a preliminary report

This is a preliminary report on the karyotype analysis of three freshwater crayfish species (Astacus astacus, A. leptodactylus and Austropotamobius torrentium) belonging to family Astacidae, all native to Croatian freshwater habitats. Chromosome number and heterochromatin distribution was studied on metaphase spreads of mitotic and meiotic cells of testis tissue. Chromosomes were stained with 4, 6-diamino-2-phenylindole stain (DAPI) specific for AT rich regions. Preliminary results showed that the chromosome number ranged from 2n=164 for Austropotamobius torrentium, 2n= 176 for Astacus astacus to 2n=180 for Astacus leptodactylus. In karyotype of each species AT rich heterochromatin blocks were observed in centromere region of all chromosomes. Terminal heterochromatin was not observed. Intercalary heterochromatin blocks were also observed on longer chromosome arm of some chromosomes in all three karyotypes (but only the largest ones).

Astacus astacus; Astacus leptodactylus; Austropotamobius torrentium; karyotype

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