engleski

Testosterone effect on immature rat ovaries

There are several animal models that were experimentally induced for evaluation of metabolic and endocrine pathophysiologic pathways of the polycystic ovaries syndrome. We performed a practical rat model for the syndrome using available testosterone depot oil solution that induces chronic hyperandrogenemy. The effect on ovulation cycles on immature female rats (3 weeks old) was evaluated as we injected testosteronenantat im. 0, 1 mg per 1 gram of body mass or the same amount of ricinus oil for the control group (6 groups per 6 animals). The injections were repeated weekly with corrected dose according to the body weight. The groups of rats were sacrificed after 3, 4 and 5 weeks of treatment. Blood samples were obtained for the progesterone serum values and ovaries were obtained for pathohystologic evaluation. The experiment confirmed that chronic hyperandrogenemy induces the formation of polycystic ovaries. Pathohystology revealed apoptosis of preantral follicles and formation of follicular cysts with apoptosis in stratum granulosum without development of corpora lutea. The serum progesterone values were significantly lower in comparison to control animals (р <0, 05). Induced hyperandrogenemy revealed pathohystologic result of typical polycystic ovaries compared to humans. The low progesterone serum values confirmed chronic anovulation. We invented this model for further experimental analysis of the pathophysiology of the syndrome.

Polycystic ovary ; Testosterone ; Immature female rat

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