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Relationship between vascular endothelial growth factor and nuclear factor-kB in renal cell tumors


Đorđević, Gordana; Matušan-Ilijaš, Koviljka; Sinožić, Emina; Damante, Giuseppe; Fabro, Dora; Grahovac, Blaženka; Lučin, Ksenija; Jonjić, Nives
Relationship between vascular endothelial growth factor and nuclear factor-kB in renal cell tumors // Croatian Medical Journal, 49 (2008), 5; 608-617 doi:10.3325/cmj.2008. (međunarodna recenzija, članak, znanstveni)


Naslov
Relationship between vascular endothelial growth factor and nuclear factor-kB in renal cell tumors

Autori
Đorđević, Gordana ; Matušan-Ilijaš, Koviljka ; Sinožić, Emina ; Damante, Giuseppe ; Fabro, Dora ; Grahovac, Blaženka ; Lučin, Ksenija ; Jonjić, Nives

Izvornik
Croatian Medical Journal (0353-9504) 49 (2008), 5; 608-617

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Vascular endothelial growth factor; renal-cell carcinoma; NF-kB

Sažetak
Aim: To assess the relationship between protein and messenger RNA (mRNA) levels of vascular endothelial growth factor (VEGF) and subcellular localization of nuclear factor-kappa B (NF-kappaB), proliferation rate of tumor cells, and clinicopathological characteristics of renal cell tumors. Methods: We analyzed 31 one renal cell tumors - 22 clear cell renal cell carcinomas (CCRCC) and 9 other histologic types (non-CCRCC). VEGF expression and subcellular localization of p65 member of NF-kappaB and Ki67 were immunohistochemically evaluated for the proliferation rate of tumor cells. Expression of VEGF mRNA was assessed using quantitative real-time polymerase chain reaction after total RNA extraction from snap-frozen tumor tissue samples. Results: Cytoplasmic localization of VEGF protein in renal cell tumors showed a perimembranous and diffuse pattern, the former being more evident in CCRCC (27.1 -/+ 18.9 vs 3.3 -/+ 10 % tumors, P<0.001) and the latter in non-CCRCC type (71.7 -/+ 23.2 vs 31.1 -/+ 22.1 % tumors, P<0.001). Heterogeneity in VEGF gene expression was more pronounced in CCRCC type than in non-CCRCC type (P=0.004). In addition, perimembranous VEGF pattern was associated with higher VEGF mRNA levels (P=0.006) and diffuse VEGF pattern with lower VEGF mRNA levels (P<0.001). Nuclear and cytoplasmic staining of NF-kappaB/p65 was observed in the majority of tumor cells. A significant association was recorded between cytoplasmic NK-kappaB/65 staining and VEGF staining of diffuse pattern (P=0.026). Association between NF-kappaB/65 and proliferation rate of tumor cells was significant for cytoplasmic staining (P=0.039) but not for nuclear NFkB/p65 staining (P=0.099). Conclusion: Higher but inhomogeneous expression of VEGF in tumor cells, especially in CCRCCs, is associated with NF-kappaB/65 activity. This indicates that both VEGF and NF-kappaB/65 may be important in renal carcinogenesis, representing a possible molecular target in the treatment of renal cell carcinoma.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti



POVEZANOST RADA


Projekt / tema
062-0620095-0078 - Uloga osteopontina u progresiji tumora (Ksenija Lučin, )
062-0620095-0082 - REGULACIJSKI ČIMBENICI ANGIOGENEZE U PROGNOZI TUMORA (Nives Jonjić, )

Ustanove
Medicinski fakultet, Rijeka

Citiraj ovu publikaciju

Đorđević, Gordana; Matušan-Ilijaš, Koviljka; Sinožić, Emina; Damante, Giuseppe; Fabro, Dora; Grahovac, Blaženka; Lučin, Ksenija; Jonjić, Nives
Relationship between vascular endothelial growth factor and nuclear factor-kB in renal cell tumors // Croatian Medical Journal, 49 (2008), 5; 608-617 doi:10.3325/cmj.2008. (međunarodna recenzija, članak, znanstveni)
Đorđević, G., Matušan-Ilijaš, K., Sinožić, E., Damante, G., Fabro, D., Grahovac, B., Lučin, K. & Jonjić, N. (2008) Relationship between vascular endothelial growth factor and nuclear factor-kB in renal cell tumors. Croatian Medical Journal, 49 (5), 608-617 doi:10.3325/cmj.2008..
@article{article, year = {2008}, pages = {608-617}, DOI = {10.3325/cmj.2008.}, keywords = {vascular endothelial growth factor, renal-cell carcinoma, NF-kB}, journal = {Croatian Medical Journal}, doi = {10.3325/cmj.2008.}, volume = {49}, number = {5}, issn = {0353-9504}, title = {Relationship between vascular endothelial growth factor and nuclear factor-kB in renal cell tumors}, keyword = {vascular endothelial growth factor, renal-cell carcinoma, NF-kB} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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