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Interferencije svojstvene kvantitativnim imunokemijskim metodama (CROSBI ID 147625)

Prilog u časopisu | pregledni rad (stručni)

Dodig, Slavica Interferences in quantitative immunochemical methods / Interferencije svojstvene kvantitativnim imunokemijskim metodama // Biochemia medica, 19 (2009), 1; 50-62

Podaci o odgovornosti

Dodig, Slavica

hrvatski

Interferencije svojstvene kvantitativnim imunokemijskim metodama

In the immunoassays, an antibody used as a reagent, detects ananlyte (antigen) of interest. Although the noncovalent bound between analyte and complementary antibody is specific, false-positive and false-negative interferences are possible. Some interferences are similar to those in chemical analyses and some are typical only for immunoassays. One should suspect interferences in following cases: upon receiving an unacceptable result, if there is non-linearity during dilution, if there is no agreement with other test results or clinical data, if different immunoassays in determination of the same analyte provide significantly different results. This paper reviews some of the possible interferences: 1) cross-reactivity with endogenous and exogenous non antibody-structured substances ; 2) cross-reactivity with endogenous and exogenous antibody-structure substances ; 3) the hook effect ; and 4) the matrix effect. By knowing and recognizing interferences in immunoassays, one can avoid possible undesired consequences: diagnostic errors, treatment and monitoring of its efficacy, unnecessary additional laboratory testing, unnecessary therapy.

imunokemijske metode ; intererencije ; križna reaktivnost ; prozonski učinak

nije evidentirano

engleski

Interferences in quantitative immunochemical methods

In the immunoassays, an antibody used as a reagent, detects ananlyte (antigen) of interest. Although the noncovalent bound between analyte and complementary antibody is specific, false-positive and false-negative interferences are possible. Some interferences are similar to those in chemical analyses and some are typical only for immunoassays. One should suspect interferences in following cases: upon receiving an unacceptable result, if there is non-linearity during dilution, if there is no agreement with other test results or clinical data, if different immunoassays in determination of the same analyte provide significantly different results. This paper reviews some of the possible interferences: 1) cross-reactivity with endogenous and exogenous non antibody-structured substances ; 2) cross-reactivity with endogenous and exogenous antibody-structure substances ; 3) the hook effect ; and 4) the matrix effect. By knowing and recognizing interferences in immunoassays, one can avoid possible undesired consequences: diagnostic errors, treatment and monitoring of its efficacy, unnecessary additional laboratory testing, unnecessary therapy.

immunoassays ; interferences ; cross-reactivity ; prozone effect

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

19 (1)

2009.

50-62

objavljeno

1330-0962

1846-7482

Povezanost rada

Farmacija, Kemija

Poveznice
Indeksiranost