A simple and reliable method for monitoring platelet serotonin levels in rats (CROSBI ID 147581)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Jernej, Branimir ; Čičin-Šain, Lipa ; Iskrić, Sonja
engleski
A simple and reliable method for monitoring platelet serotonin levels in rats
A simple and reliable method for individual monitoring of platelet serotonin in rats is developed. Platelet-rich plasma is prepared under standardized conditions from 1 mL of venous blood and the platelets are quantitatively separated by a highly reproducible procedure. Platelet serotonin content is determined spectrophotofluorometrically and the results are comparatively expressed per standardized platelet rich plasma sample (1.01 ± ; 0.18 μ g), per mg of platelet protein (1.57 ± ; 0.15 μ g) and per 109 platelets (2.16 ± ; 0.38 μ g). Normal distribution of platelet serotonin levels in a sample of 338 animals is shown. By use of the described method, the intraindividual stability of platelet serotonin concentration in rats is demonstrated for the first time.
serotonin ; platelet ; rat
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nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o izdanju
Povezanost rada
Biologija, Temeljne medicinske znanosti