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Pregled bibliografske jedinice broj: 380348

Nm23-H1 promotes adhesion of CAL 27 cells in vitro


Bago, Ružica; Pavelić, Jasminka; Maravić Vlahoviček, Gordana; Herak Bosnar, Maja
Nm23-H1 promotes adhesion of CAL 27 cells in vitro // Molecular carcinogenesis, 48 (2009), 9; 779-789 doi:10.1002/mc.20536 (međunarodna recenzija, članak, znanstveni)


Naslov
Nm23-H1 promotes adhesion of CAL 27 cells in vitro

Autori
Bago, Ružica ; Pavelić, Jasminka ; Maravić Vlahoviček, Gordana ; Herak Bosnar, Maja

Izvornik
Molecular carcinogenesis (0899-1987) 48 (2009), 9; 779-789

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Adhesion ; CAL 27 ; migration ; nm23 ; OSCC

Sažetak
Nm23-H1 was found to diminish metastatic potential of carcinoma cell lines and therefore was placed in the group of metastatic suppressor genes. Its protein product has a function of a nucleosid-diphosphate kinase (NDPK) as well as protein kinase and nuclease. Though it was found that nm23-H1 is involved in many cellular processes, it is still not known how it promotes metastatic suppressor activity. Since the process of metastasis is dependent on adhesion properties of cells, the goal of our work was to describe the adhesion properties of CAL 27 cells (oral squamous cell carcinoma of the tongue) overexpressing FLAG/nm23-H1. In our experiments, cells overexpressing nm23-H1 show reduced migratory and invasive potential. Additionally, cells overexpressing Nm23-H1 adhere stronger on substrates (collagen IV and fibronectin) and show more spread morphology than the control cells. Results obtained by EGF induction of migration revealed that the adhesion strength predetermined cell response to chemoattractant and that Nm23-H1, in this cell type, does not interfere with, EGF induced, Ras signaling pathway. These data contribute to the overall knowledge about nm23-H1 and its role in cell adhesion, migration and invasion, especially in oral squamous cell carcinoma.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
098-0982464-2394 - Gensko liječenje tumora djelovanjem na molekule imunološkog sustava (Jasminka Pavelić, )
098-0982464-2513 - Uloga gena nm23 u karcinomu pločastog epitela usne šupljine (Maja Herak-Bosnar, )
101-0982464-2277 - Molekularno profiliranje metastazirajućeg tumora dojke (Dragan Dekaris, )

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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