Effects of pioglitazone on the lipid peroxidation levels and COX-2 expressions in different brain structures of rats with lithium plus pilocarpine- induced status epilepticus (CROSBI ID 544969)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Župan, Gordana ; Peternel, Sandra ; Pilipović, Kristina ; Blagaić, Ana ; Hrelja, Ana ; Vitezić, Dinko
engleski
Effects of pioglitazone on the lipid peroxidation levels and COX-2 expressions in different brain structures of rats with lithium plus pilocarpine- induced status epilepticus
In our previous study we demonstrated that pioglitazone, a synthetic agonist of the peroxisome proliferator-activated receptors (PPAR) gamma, causes the prominent increase of the 24 h- survival rate in rats with lithium plus pilocarpine-induced status epilepticus (SE), that provides a common experimental model of temporal lobe epilepsy (TLE) in humans. With the aim to investigate possible mechanisms underlying mentioned drug effect, we determined the lipid peroxidation levels and COX-2 expressions in some brain structures of rats with SE and treated by pioglitazone. Experiments were performed on 80 to 90 days old male Wistar rats. Pioglitazone (1 ; 3 mg/kg) or vehicle were administered i.p. 10 min after the lithium plus pilocarpine-induced SE, that was interrupted 2 h after its onset by an injection of diazepam. Rats were sacrificed 24 h after the SE onset and Western blotting analyses of COX-2 expressions were performed in the piriform/entorhinal cortex, temporal neocortex and in the hippocampus. Levels of the brain lipid peroxidation were determined by thiobarbituric acid reactive substances (TBARS) assay. Lithium plus pilocarpine-induced SE caused increases in the lipid peroxidation levels and COX-2 overexpressions in all examined brain regions. In rats with SE and treated by pioglitazone, TBARS levels were reduced in the piriform/entorhinal cortex and in the temporal neocortex. COX-2 overexpression was decreased in the hippocampus. Conclusion: Our results suggest that pioglitazone could be a candidate drug to protect examined brain structures in patients with TLE.
status epilepticus; oxidative stress; cyclooxygenase-2; pioglitazone; rats
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Podaci o prilogu
307-307.
2008.
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objavljeno
Podaci o matičnoj publikaciji
European journal of neurology
Lenzi, Gian Luigi ; Gil-Nagel, Antonio
Madrid: Wiley-Blackwell
1351-5101
Podaci o skupu
Congress of the European Federation of Neurological Societies (12)
poster
23.08.2008-26.08.2008
Madrid, Španjolska
Povezanost rada
Temeljne medicinske znanosti