engleski

Glycosphingolipid expression in neural tissues of three rat strains

Many neurological disorders and injuries such as Parkinson's disease, multiple sclerosis, stroke, traumatic injury to CNS etc. have movement abnormalities as major symptom. Numerous animal models of neurological disease and injury exist, especially in rats, and most common used strains are Lewis, Wistar and Sprague-Dawley (SD). These three strains show different neurological behaviour. Glycosphingolipids (GSLs), both neutral and gangliosides are, according to their role, differently distributed in neural tissues and synaptic mechanisms. Therefore, comparison of GSL expression in neural tissues of three rat strains was performed. The aim was to identify GSL profile in brain, cerebellum and brain stem of each rat strain which could be responsible for different neurological behaviour. Three different male, age-matched rat strains were used: Lewis, Wistar and Sprague-Dawley. GSLs were isolated from animal tissues by standard procedure. Distribution of GSLs in brain, cerebellum and brain stem of each group of animals were analyzed by orcinol staining. GSLs were abundant in the brain of Lewis rats compared to lower expression in Wistar brain and traces of GSLs in SD brain. Cerebellum and brain stem of Wistar rats showed the highest GSL expression. Lewis cerebellum and brain stem showed lower expression of gangliosides and higher of neutral GSLs compared to the same Wistar tissues. SD cerebellum and brain stem showed traces of gangliosides and abundant expression of neutral GSLs. We define GSL profile in brain, cerebellum and brain stem of f Wistar, Lewis and SD rats which could be starting point for choice and defining neurochemical experiments with those strains.

glycosphingolipid; neural tissues; rat strains

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