The use of the hypoosmotic swelling test and supravital staining in evaluation of sperm quality in boars (CROSBI ID 146456)
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Samardžija, Marko ; Dobranić, Tomislav ; Krušlin, Suzana ; Cergolj, Marijan ; Karadjole, Martina ; Prvanović, Nikica ; Grizelj, Juraj
engleski
The use of the hypoosmotic swelling test and supravital staining in evaluation of sperm quality in boars
Semen samples from ten boars, Yorkshire and Pietrain breed, aged between two and six years were used. The sperm quality was evaluated using the following parameters: the motility (subjective), the structural supravital staining (eosine/nigrosine) and the functional integrity of sperm membrane (HOS test). The majority of HOS reactive or swelled up spermatozoa were gained in a hypoosmolar solution of 100 mOsm/kg by incubation of 30 minutes. Between middle values for HOS reactive (swollen up) spermatozoa in hypoosmolar solutions with different osmolarity, significant differences (P<0.05) were determined. Using a hypoosmotic solution with different osmolarity 100 mOsm/kg, significantly strong, positive correlations between HOS and motility (r = 0.98 ; P<0.05) were determined, between HOS test and supravital staining eosine/nigrosine (r = 0.99 ; P<0.05) and between motility and supravital staining eosine/nigrosine (r = 0.98 ; P<0.05). The results of our research have shown that the HOS test and supravital staining eosine/nigrosine could be used for routine evaluation of the functional and structural integrity of diluted boar sperm.
boar ; semen ; hypoosmotic test ; eosine/nigrosine staining
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