THE EFFECT OF SECONDARY METABOLITES ISOLATED FROM INDOOR MICROMYCETES ON LUNG CELLS - IN VITRO STUDY (CROSBI ID 146112)
Prilog u časopisu | kratko priopćenje
Podaci o odgovornosti
Kováčiková, Z ; Tátrai, E ; Mataušic-Pišl Mirjana ; Jančinová, V ; Piecková, E ; Kolláriková, Z ; Wsólová L
engleski
THE EFFECT OF SECONDARY METABOLITES ISOLATED FROM INDOOR MICROMYCETES ON LUNG CELLS - IN VITRO STUDY
The indoor pollution in buildings contaminated with micromycetes has specific components: spores and secondary metabolites from micromycetes. The effect of endo- and exometabolites from micromycetes isolates (Aspergillus ustus, Aspergillus versicolor, Penicillium chrysogenum, Stachybotrys chartarum) were studied in vitro on isolated lung cells. The toxicological most important types of lung cells were used in this study: alveolar macrophages (AM), alveolar epithelial type II cells (isolated from rats) and non-ciliated bronchiolar Clara cells (isolated from mice). The isolated cells were cultivated with fungal isolates, the cultivation was terminated after 24 h. The cytotoxicity was evaluated in cultivation medium by ToxiLight BioAssay Kit, the activity of marker enzymes was estimated: acid phosphatase in alveolar macrophages, alkaline phosphatase in type II cells. The changes on the cell surface of type II cells were demonstrated by staining with Maclura pomifera lectin. The control cells showed positive staining of membranes whilst the staining of cells cultured with metabolites revealed fragmentation of the membranes. The activities of enzymes in cells decreased with enhanced concentration of metabolites. The cytotoxic effect was confirmed in medium. The results showed toxic effect of all tested endo- and exo-metabolites even in very low concentration (0.1 μ g/ml).
indoor pollution; clara cells; cytotoxicity
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano