Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 37492

Oncogene activation and apoptosis as possible mechanism of antitumour effect of ferric-sorbitol-citrate


Poljak-Blaži, Marija; Kralj, Marjeta; Popović Hadžija, Marijana
Oncogene activation and apoptosis as possible mechanism of antitumour effect of ferric-sorbitol-citrate // Proceedings of the 10th International Symposium on Trace Elements in Man and Animal (TEMA 10) / Roussel, A.M. ; Anderson, R.A. ; Favier, A.E. (ur.).
New York: Cluwer Academic/Plenum Publischer, 2000. str. 123-126 (predavanje, međunarodna recenzija, cjeloviti rad (in extenso), znanstveni)


CROSBI ID: 37492 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Oncogene activation and apoptosis as possible mechanism of antitumour effect of ferric-sorbitol-citrate

Autori
Poljak-Blaži, Marija ; Kralj, Marjeta ; Popović Hadžija, Marijana

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Proceedings of the 10th International Symposium on Trace Elements in Man and Animal (TEMA 10) / Roussel, A.M. ; Anderson, R.A. ; Favier, A.E. - New York : Cluwer Academic/Plenum Publischer, 2000, 123-126

Skup
International Symposium on Trace Elements in Man and Animal (10 ; 1999)

Mjesto i datum
Evian, Francuska, 02.-07.05.1999

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Jectofer; tumour cell proliferation; apoptosis; oncogenes

Sažetak
The complex mechanism of Jectofer influence on cell proliferation is not entirely understood. For a better understanding of the mechanism of drug action, DNA fragmentation in control and treated CaCo2 (colon carcinoma) cells was analised by 1.5% agarose gel electrofores. Influence of iron from FSC, on the expression of c-myc, Bcl-2, and wild and mutant p53 protein was also examined. After treatment, for 72 hours, with ferric sorbitol citrate (FSC) we obserwed impaired viability of three carcinoma (Ca) cell line ; CaCo2 (colon Ca), MiaPaCa2 (pancreatic Ca) and Hela (cervics Ca), measured by MTT assay. The most pronounced growth inhibitory effect was observed on MiaPaCa and CaCo2 cells. After treatment with two higher doses of FSC (10-4 to 10-3 M), about 70 % of viable cells left. In spite of that, all concentrations of FSC stimulated the proliferation of non malignant HEF522 (human embrionic fibroblasts) cells. Intensity of apoptotic processes was greater in treated cells than in non treated CaCo2 cells. The degree of DNA fragmentation followed the morphological changes and the growth inhibition of the investigated cells. Growth modification of CaCo2 cells caused by FSC involves interference with the diminished expression of Bcl-2, and an over expression of mp53 oncogene. Expression of c-my and wp53 protein was not altered after tretment with FSC. Thus, the specific antitumour effect of FSC involves DNA damage, oncogene alteration, and apoptosis. The mechanism of FSC-activity might result in dual activity of this iron containing drug: inhibition of malignant cell proliferation and induction of apoptosis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
00981104

Ustanove:
Institut "Ruđer Bošković", Zagreb


Citiraj ovu publikaciju:

Poljak-Blaži, Marija; Kralj, Marjeta; Popović Hadžija, Marijana
Oncogene activation and apoptosis as possible mechanism of antitumour effect of ferric-sorbitol-citrate // Proceedings of the 10th International Symposium on Trace Elements in Man and Animal (TEMA 10) / Roussel, A.M. ; Anderson, R.A. ; Favier, A.E. (ur.).
New York: Cluwer Academic/Plenum Publischer, 2000. str. 123-126 (predavanje, međunarodna recenzija, cjeloviti rad (in extenso), znanstveni)
Poljak-Blaži, M., Kralj, M. & Popović Hadžija, M. (2000) Oncogene activation and apoptosis as possible mechanism of antitumour effect of ferric-sorbitol-citrate. U: Roussel, A., Anderson, R. & Favier, A. (ur.)Proceedings of the 10th International Symposium on Trace Elements in Man and Animal (TEMA 10).
@article{article, author = {Poljak-Bla\v{z}i, Marija and Kralj, Marjeta and Popovi\'{c} Had\v{z}ija, Marijana}, year = {2000}, pages = {123-126}, keywords = {Jectofer, tumour cell proliferation, apoptosis, oncogenes}, title = {Oncogene activation and apoptosis as possible mechanism of antitumour effect of ferric-sorbitol-citrate}, keyword = {Jectofer, tumour cell proliferation, apoptosis, oncogenes}, publisher = {Cluwer Academic/Plenum Publischer}, publisherplace = {Evian, Francuska} }
@article{article, author = {Poljak-Bla\v{z}i, Marija and Kralj, Marjeta and Popovi\'{c} Had\v{z}ija, Marijana}, year = {2000}, pages = {123-126}, keywords = {Jectofer, tumour cell proliferation, apoptosis, oncogenes}, title = {Oncogene activation and apoptosis as possible mechanism of antitumour effect of ferric-sorbitol-citrate}, keyword = {Jectofer, tumour cell proliferation, apoptosis, oncogenes}, publisher = {Cluwer Academic/Plenum Publischer}, publisherplace = {Evian, Francuska} }




Contrast
Increase Font
Decrease Font
Dyslexic Font