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Physiological responses in two radish cultivars exposed to Copper and Lead stress

Shallow-rooted vegetable species such as radish (Raphanus sativus L.) are often grown in urban and suburban areas, where garden soils might be polluted through long-lasting exposure to traffic-induced Pb accumulation and by the application of Cu-based fungicides. This investigation showed that the exposure of two radish cultivars (Saxa 2 and Saxa Treib) to 0.5 mM copper or lead in nutrient solution for only two days, significantly inhibited plant growth, stimulated lipid peroxidation and elicited the antioxidative response in radish hypocotyls and leaves. The established connections among guaiacol peroxidase and catalase total or specific activities, protein and free proline content in radish tissues, indicate synergistic effect of enzymatic and non-enzymatic antioxidative mechanisms in this plant species. The interactions of plant part, cultivar and heavy metal treatment, influenced significantly on tested parameters of plant response to copper and lead toxicity. It has to be emphasized that a significantly different assessment of plant antioxidative response can be obtained if defined by total or specific enzyme activity, depending also on plant part and genotype. Regardless of genotype and heavy metal applied, very significant positive linear correlations were established between guaiacol peroxidase and catalase total activities in hypocotyl and their specific activities in leaves.

Copper ; heavy metal stress ; Lead ; oxidative stress ; radish

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