Molecular structure of spinach chloroplast nucleoids (CROSBI ID 85891)
Prilog u časopisu | ostalo
Podaci o odgovornosti
Fulgosi, Hrvoje ; Ljubešić, Nikola
engleski
Molecular structure of spinach chloroplast nucleoids
The ultrastructure of molecular composition of spinach chloroplast nucleoids were studied. Isolated nucleoids retained their thre-dimensionally folded stucture observed in situ. Fluorescence microscopy and staining with DAPI reaffirmed the mostly DNA-containing nature of these complexes. The presence of chloroplast DNA was confirmed by restriction fragment analysis. Electron microscopy revealed a highly condensed, chromatin-like, beaded structure. Naked DNA supercoils or loops were not observed. Integral nucleoid proteins were isolated and analysed on SDS-polyacrylamide gels. A distinct pattern of the nucleoid proteins, covering the entire range of molecular masses from 5 kDa to > 100 kDa revealed that the nucleoid fraction was highly depleted of major thylakoid and stroma constituents.
spinach; chloroplast nucleoids; cpDNA; proteins; microscopy
nije evidentirano
hrvatski
Molekularna struktura kloroplastnih nukleoida špinata (Spinacea oleracea)
Istraživali smo ultrastrukturu i molekularnu građu kloroplastnih nukleoida špinata (Spinacea oleráceo). Izolirani nukleoidi zadržavaju svoju trodimenzionalnu građu vidljivu in situ. Fluorescencijskom mikroskopijom i bojenjem s fluorokromom DAPI potvrdili smo visok sadržaj DNA u ovim kompleksima. Prisustvo kloroplastne DNA dokazali smo restrikcijskom analizom. Pod elektronskim mikroskopom vidljiva je kondenzirana (gusta), kromatinu slična, kugličasta građa. Gole DNA petlje ili superzavojnice nisu primjećene. Proteini nukleoida pokrivaju čitav raspon molekulskih masa, od 5 do više od 100 kDa. Glavni proteini tilakoidnih membrana i strome kloroplasta nisu prisutni u nukleoidnoj frakciji.
špinat; kloroplastni nukleoidi
nije evidentirano
