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Pregled bibliografske jedinice broj: 373870

Proposed arrangement of proteins forming a bacterial type II polyketide synthase


Castaldo, Gaetano; Žučko, Jurica; Heidelberger, Sibylle; Vujaklija, Dušica; Hranueli, Daslav; Wattana-Amorn, Pakorn; Crump, P. Matthew; Crosby, John; Long, F. Paul
Proposed arrangement of proteins forming a bacterial type II polyketide synthase // Chemistry & biology, 15 (2008), 11; 1156-1165 doi:10.1016/j.chembiol.2008.09.010 (međunarodna recenzija, članak, znanstveni)


Naslov
Proposed arrangement of proteins forming a bacterial type II polyketide synthase

Autori
Castaldo, Gaetano ; Žučko, Jurica ; Heidelberger, Sibylle ; Vujaklija, Dušica ; Hranueli, Daslav ; Wattana-Amorn, Pakorn ; Crump, P. Matthew ; Crosby, John ; Long, F. Paul

Izvornik
Chemistry & biology (1074-5521) 15 (2008), 11; 1156-1165

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Aklanonic acid; daunorubicin; doxorubicin; type II polyketide synthase; protein structure

Sažetak
Aklanonic acid is synthesised on a type II polyketide synthase (PKS) composed of 8 proteins that catalyze the condensation between a propionate starter unit and 9 malonate extender units. We have investigated the network of protein interactions within this complex using a yeast two hybrid system, coaffinity chromatography and by computer aided protein docking simulations. Our results suggest that the ketosynthase (KS) α and β subunits interact with each other and that the KSα subunit (DpsA) also probably interacts with a malonyl-CoA: ACP acyltransferase (DpsD) forming a putative minimal synthase. We speculate that DpsD may physically inhibit the priming reaction allowing the choice of propionate rather than acetate as the starter unit. We also suggest a structural role for the cyclase (DpsY) maintaining the overall structural integrity of the complex.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija



POVEZANOST RADA


Projekt / tema
058-0000000-3475 - Generiranje potencijalnih lijekova u uvjetima in silico (Daslav Hranueli, )
098-0982913-2877 - Temeljna molekularno-biološka istraživanja streptomiceta (Dušica Vujaklija, )

Ustanove
Prehrambeno-biotehnološki fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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