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Pregled bibliografske jedinice broj: 373708

Influence of curing mode intensities on cell culture cytotoxicity/genotoxicity


Knežević, Alena; Želježić, Davor; Kopjar, Nevenka; Tarle, Zrinka
Influence of curing mode intensities on cell culture cytotoxicity/genotoxicity // American Journal of Dentistry, 22 (2009), 1; 43-48 (međunarodna recenzija, članak, znanstveni)


Naslov
Influence of curing mode intensities on cell culture cytotoxicity/genotoxicity

Autori
Knežević, Alena ; Želježić, Davor ; Kopjar, Nevenka ; Tarle, Zrinka

Izvornik
American Journal of Dentistry (0894-8275) 22 (2009), 1; 43-48

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cytotoxicity; genotoxicity; curing modes; temperature

Sažetak
Purpose: Light curing units with different light intensity or different curing modes cause different temperature increase during composite resin polymerization. Heat generated during composite resin polymerization can cause irreversible damage of vital pulp tissue. Therefore, the aim of this study was to determine the cytotoxic/genotoxic effect of different curing modes on cell culture. Methods: A thin layer of lymphocyte cultures was cured applying three different curing modes of Bluephase C8 LED curing unit (Vivadent, Schaan, Liechtenstein, ). Cultures were exposed to light directly or through a layer (2 mm) of polymerized composite resin sample. Cells were analyzed using trypan blue exclusion test, acridine orange/ethidium bromide dying technique, and alkaline comet assay. Results: Only low intensity mode (LOP) after direct exposure significantly increased the number of nonviable lymphocytes detected using trypan blue. All curing procedures significantly increased the number of apoptotic lymphocytes regardless whether the exposure occurred directly or through the composite. LOP in direct exposure significantly elevated DNA migration compared to other curing modes. One hour after exposure significant increase in tail length and intensity for all modes and procedures was detected. However, DNA damage measured for cultures cured by LOP was higher compared to other two modes ; thus, despite of curing light intensity, longer curing time leads to greater cytotoxicity/genotoxicity in cell culture.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina



POVEZANOST RADA


Projekt / tema
022-0222148-2137 - Genotoksičnost kemijskih i fizikalnih agensa prirodnog i antropogenog podrijetla (Vilena Kašuba, )
065-0352851-0410 - Nanostruktura restaurativnih materijala i interakcije s tvrdim zubnim tkivima (Zrinka Tarle, )

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Stomatološki fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE