engleski

DNA polymerase chain reaction in diagnosis of human herpes simplex virus

Traditional methods for the detection of pathogenesis include visual identification under the microscope, culture and growth characteristics of an organism recognition of antigenic determinants related to the organism and the host's specific immune responses, i.e. production of antibodies. However, direct visualisation or culture is not always possible. These techniques can also be time-consuming and tecnically difficult. In the long term, many of the conventional diagnostic approaches will be complemented or even replaced by detection of DNA/RNA-specific sequences through nucleic acid hybridisation techniques or DNA amplification by polymerase chain reaction has emerged rapidly as a front runner for the identification and study of pathogens. Automation is available and the technology is less demanding if probe labelling and hybridisation steps are not required. Multiplex DNA amplifications will increase cost-effectiveness by enabling simultaneous detection of a number of pathogens. A balance between what is possible by traditional approaches and what DNA offer will enable the establishment of priorities to identify which pathogens are best studied by recombinant DNA means. Recombinant DNA approaches have now been described for detection of wide range of infectious agents. The author will discuss present possibilities and the use of DNA-PCR for human herpes virus detection both in the routine diagnostic laboratory and for research activities. DNA amplification by the polymerase chain reaction is technology which has a real potential to change significantly the future approach to the practice of medical microbiology paricularly in relation to the routine diagnostic laboratory. A lot of research laboratories have incorporated DNA technology into a number of their protocols. The prospects for molecular medicine in microbiology are vast and will have profound long-term effects in laboratory and clinical practice.

PCR; HSV

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