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Pregled bibliografske jedinice broj: 370755

Identification of a novel HPV 16 E1 gene variant


Sabol, Ivan; Matovina, Mihaela; Grce, Magdalena
Identification of a novel HPV 16 E1 gene variant // Program & Abstract Book of the 5th International HPV & Skin Cancer Conference, Heidelberg, Germany, 22-24 October 2008 / Gissmann, Lutz ; Nindle, Ingo ; Rosl, Frank (ur.).
Heidelberg: DKFZ, 2008. str. 9-9 (pozvano predavanje, međunarodna recenzija, sažetak, znanstveni)


Naslov
Identification of a novel HPV 16 E1 gene variant

Autori
Sabol, Ivan ; Matovina, Mihaela ; Grce, Magdalena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Program & Abstract Book of the 5th International HPV & Skin Cancer Conference, Heidelberg, Germany, 22-24 October 2008 / Gissmann, Lutz ; Nindle, Ingo ; Rosl, Frank - Heidelberg : DKFZ, 2008, 9-9

Skup
Human papillomavirus and cancer. 5th International HPV & Skin Cancer Conference, Heidelberg, Germany, 22-24 October 2008

Mjesto i datum
Heidelberg, Njemačka, 22-24.10.2008

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Human papillomavirus type 16 variant

Sažetak
The Human papillomavirus (HPV) 16 genome has been extensively studied, although no study has focused on the E1 gene that is implicated in viral DNA replication. After analyzing the E1 region of HPV 16 genomes in 429 cervical specimens, 11.2% were found to contain 63-nucleotides duplication in this region. Sequence analysis of the E6 and the E7 regions has shown that all samples containing this duplication were related to E6-G350 variant of the HPV 16. In silico translation of the E1 gene with the duplication has shown that the protein has extra 21 amino acids as the duplication is “ in frame” and does not introduce a premature stop codon. The affected region has currently no significant function ascribed to it. However, comparison of cervical lesion severity of the examinees having normal or variant E1 genes has shown that the variant group is significantly (Fischers exact test, P=0.0401) less associated to high grade cervical lesions, suggesting that this particular duplication might reduce the oncogenic potential of HPV 16, and also might clarify the differences of E6-G350 variant oncogenicity observed in European populations. Further research is needed to elucidate the biology of these HPV 16 E1 variants and the biological effect of this particular duplication.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

Napomena
Istovremeno i poster prezentacija



POVEZANOST RADA


Projekt / tema
098-0982464-2510 - Promijenjeno stanje DNA-metilacije u HPV-povezanim oštećenjima (Magdalena Grce, )

Ustanove
Institut "Ruđer Bošković", Zagreb