engleski

Activity of glutatitone peroxidase in cattle blood under different storage conditions

The purposed was to determine glutathione peroxidase (GSH-Px)acitivities in samples of whole blood of fattening yearlings under differen storage coditions. Blood samples from V. jugularis were taken freom seven healthy male animals aged 6-8 months into Vacutainer epruvettes. Each samples was separeted into nine Eppedorf epruvettes. Four samples from each animal were stored in a refrigerator at 4C, while five samples were stored in a freezer at -20C. The glutathion peroxidase activity in samples wad determined immediately, and on th first, second, fourth and seventh days after the samples were taken. In samples stored at -20C activity was also detemined on the 15th day after sampling taken. GSH-Px acitivity was determined by the RANSEL (Randox- Laboratories) method. The average value of enzyme activity increased during the first seven days of measuring in all tested samples (P>0.05), althought it is pointed out that the increase was lower in the frozen samples. Also, on the fifteenth day, GSH-Px acitivity in samples stored at -20C returned to its starting values (P<0.05). The study suggests to detemine glutathion peroxidase activity within two days after collection in samples stored at 4C, and within 15 days in frozen samples.

glutathione peroxidase; blood; yearling cattle

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