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Pregled bibliografske jedinice broj: 370021

Clenbuterol residues in plasma and urine samples of food-producing pigs during and after subchronic exposure to a growth-promoting dose


Pleadin, Jelka; Gojmerac, Tihomira; Bratoš, Igor; Lipej, Zoran; Novosel, Dinko; Vulić, Ana
Clenbuterol residues in plasma and urine samples of food-producing pigs during and after subchronic exposure to a growth-promoting dose // Food Technology and Biotechnology, 47 (2009), 1; 67-74 (međunarodna recenzija, članak, znanstveni)


Naslov
Clenbuterol residues in plasma and urine samples of food-producing pigs during and after subchronic exposure to a growth-promoting dose

Autori
Pleadin, Jelka ; Gojmerac, Tihomira ; Bratoš, Igor ; Lipej, Zoran ; Novosel, Dinko ; Vulić, Ana

Izvornik
Food Technology and Biotechnology (1330-9862) 47 (2009), 1; 67-74

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Clenbuterol residues; growth promoting; subchronic exposure; pig; urine; plasma

Sažetak
The aim of the study was to evaluate suitability of plasma and urine as matrices for clenbuterol residue determination during and after its subchronic administration at a growth-promoting dose to male pigs, using previously validated enzyme-linked immunosorbent assay (ELISA) as a screening method and liquid chromatography tandem mass spectrometry (LC-MS/MS) as a confirmation method. A high correlation coefficient between these analytical methods was obtained for both urine (r=0.9800) and plasma (r=0.9970) concentrations. Study results showed the plasma and urine concentration to vary greatly during oral treatment with clenbuterol for 28 days. The peak urine concentration (88.54± ; 50.54 ng/mL) recorded on day 21 was 40-fold peak plasma concentration (2.25± ; 1.54 ng/mL). After withdrawal period, the peak urine clenbuterol concentration (42.93± ; 10.52 ng/mL) recorded on day 0 was 24-fold plasma concentration (1.79± ; 0.97 ng/mL). The maximal allowed concentration of 0.5 ng/g in the liver as a regulated matrix for control of clenbuterol abuse was achieved in plasma on day 3 (0.52± ; 0.26 ng/mL) and in urine on day 7 of treatment withdrawal (0.45± ; 0.11 ng/mL). Study results indicated that urine and plasma may be suitable matrices in the control of clenbuterol abuse during food producing pig fattening but have a limited value because of the rapidly decreasing concentration upon treatment withdrawal, in plasma in particular.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita



POVEZANOST RADA


Projekt / tema
048-0531854-0467 - Ostaci kemijskih kontaminanata u hrani: Farmakokinetika i toksikologija (Dražen Oraić, )

Ustanove
Hrvatski veterinarski institut, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Uključenost u ostale bibliografske baze podataka:


  • World Aluminum Abstracts
  • Viniti
  • Vitis-Vea
  • Directory of Open Access Journals