engleski

Qualitative and quantitative parameters of swine cell immunity

Flow cytometry is a modern analytic method for qualitative and quantitive analyses of procaryotic and eukaryotic cells. Classical analytical methods examine one characteristic as an average of an examined cell population. Flow cytometry simultaneously measures several biochemical and physical parameters of every single cell in a heterogenic cell sample. Flow cytometry is routinely used all over the world in professional work of chemical laboratories in veterinarian medicine. For that purpose on Veterinary faculty of University in Zagreb, machine for flow cytometry was standardized for use in veterinarian medicine, especially for immunophenotypification of haemopoetic cells of domestic animals. In this manuscript we showed the method of standardisation on a model of swine, on samples of peripheral blood and for immunophenotypification of CD45+, CD3+ and CD21+ cells.

flow citometry; immunophenotypization; swine

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