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Structure-spectrophotometric selectivity relationship in interactions of quercetin related flavonoids with double stranded and single stranded RNA


Piantanida, Ivo; Mašić, Lozika; Rusak, Gordana
Structure-spectrophotometric selectivity relationship in interactions of quercetin related flavonoids with double stranded and single stranded RNA // Journal of molecular structure, 924– 926 (2009), 138-143 doi::10.1016/j.molstruc.2008.10.032 (međunarodna recenzija, članak, znanstveni)


Naslov
Structure-spectrophotometric selectivity relationship in interactions of quercetin related flavonoids with double stranded and single stranded RNA

Autori
Piantanida, Ivo ; Mašić, Lozika ; Rusak, Gordana

Izvornik
Journal of molecular structure (0022-2860) 924– 926 (2009); 138-143

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Flavonoids; dsRNA; ssRNA; UV/vis spectroscopy

Sažetak
Interactions of five flavonoids with dsRNA and single stranded ssRNA were studied by UV/vis titrations. The results obtained supported the intercalative binding mode as a dominant interaction of studied flavonoids with dsRNA as well as major interaction with ssRNA. Furthermore, changes of the UV/vis spectra of flavonoids induced by addition of poly G or poly C, respectively, are significantly stronger than changes induced by double stranded poly G-poly C, pointing to essential role of the free poly G or poly C sequence (not hydrogen bonded in double helix). Exclusively poly G caused significant batochromic shift of the UV/vis maxima of all studied flavonoids, whereby the intensity of batochromic shift is nicely correlated to the number of OH groups of flavonoid. Unlikely to poly G, addition of poly A and poly U induced measurable changes only in the UV/vis spectra of flavonoids characterised by no OH (galangin) or three OH groups (myricetin) on the phenyl part of the molecule. Consequently, flavonoids with one or two -OH groups on the phenyl part of the molecule (luteolin, fisetin, kaempferol) specifically differentiate between poly A, poly U (negligible changes in the UV/Vis spectra) and poly G (strong changes in the UV/Vis spectra) as well as poly C (moderate changes in the UV/Vis spectra).

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

Napomena
Zbornik radiva s 29. European Congress on Molecular Spectroscopy, održanog od 31.08.-05.09.2008, Opatija, Hrvatska ; J. Barnes, Krešimir Furić and Svetozar Musić (ur.)



POVEZANOST RADA


Projekt / tema
098-0982914-2918 - Dizajn, sinteza i ispitivanje interakcija malih molekula s DNA, RNA i proteinima (Ivo Piantanida, )
119-1191192-1213 - Flavonoidi i molekularni mehanizmi njihovih bioloških učinaka (Gordana Rusak, )

Ustanove
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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