Cytotoxicity of Ozone (CROSBI ID 542293)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Negovetić Mandić, Višnja ; Želježić, Davor ; Tarle, Zrinka ; Pandurić, Vlatko ; Matošević, Danijela ; Kopjar, Nevenka
engleski
Cytotoxicity of Ozone
Objectives: Objective of this study was to determine the degree of DNA molecule damage in different time intervals after ozone exposure. Methods: In this study alkaline comet assay cytogenetic technique modified by applying formamidopyrimidine-DNA glycosylase (Fpg) was used to enable detection of DNA damage caused by oxidative stress. To evaluate genome damage primary human lymphocyte cultures were used. Samples were then analyzed using Comet assay II automatic digital analysis system (Perceptive Instruments Ltd, Suffolk, Halstead, UK) to measure the tail moment and tail intensity (percentage of DNA that migrated into the tail). Results: In the Table 1, tail intensity and tail moment represent a degree of damage after the cells being treated with buffer without the endonuclease (labeled buffer) and the second column (labeled Fpg) represent a degree after treatment with Fpg. So, if there is an oxidative damage, the difference between parameters measured with Fpg and only buffer represent exact intensity of oxidative damage. Conclusion: Even though there is some initial DNA damage, two hours following ozone application there are no differences observed between treated and control lymphocytes, indicating that the induced lesions have been efficiently repaired. This study was supported by grant 065-0352851-0410 of MZOS, Croatia.
cytotoxicity; ozone
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
148-148.
2008.
objavljeno
Podaci o matičnoj publikaciji
PEF IADR 2008, Conference Programme and Abstracts
London : Delhi: International Association for Dental Research
Podaci o skupu
4th International Congress of the Pan European Federation of the International Association for Dental Research
poster
10.09.2008-12.09.2008
London, Ujedinjeno Kraljevstvo