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Pregled bibliografske jedinice broj: 367455

Enhanced Digestion Efficiency, Peptide Ionization Efficiency, and Sequence Resolution for Protein Hydrogen/Deuterium Exchange Monitored by FT-ICR Mass Spectrometry


Zhang, Huimin; Kazazić, Saša; Schaub, Tanner; Tipton, Jeremiah; Emmett, Mark; Marshall, Alan
Enhanced Digestion Efficiency, Peptide Ionization Efficiency, and Sequence Resolution for Protein Hydrogen/Deuterium Exchange Monitored by FT-ICR Mass Spectrometry // Analytical chemistry, 80 (2008), 23; 9034-9041 doi:10.1021/ac801417d (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 367455 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Enhanced Digestion Efficiency, Peptide Ionization Efficiency, and Sequence Resolution for Protein Hydrogen/Deuterium Exchange Monitored by FT-ICR Mass Spectrometry

Autori
Zhang, Huimin ; Kazazić, Saša ; Schaub, Tanner ; Tipton, Jeremiah ; Emmett, Mark ; Marshall, Alan

Izvornik
Analytical chemistry (0003-2700) 80 (2008), 23; 9034-9041

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Enhanced Digestion Efficiency ; Peptide Ionization Efficiency ;

Sažetak
Solution-phase hydrogen/deuterium exchange (HDX) monitored by high-resolution Fourier transform ion cyclotron resonance (FTICR) mass spectrometry offers a rapid method to study protein conformations and protein− protein interactions. Pepsin is usually used to digest proteins in HDX and is known for lack of cleavage specificity. To improve digestion efficiency and specificity, we have optimized digestion conditions and cleavage preferences for pepsin and protease type XIII from Aspergillus saitoi. A dilution series of the proteases was used to determine the digestion efficiency for several test proteins. Protease type XIII prefers to cleave on the C-terminal end of basic amino acids and produced the highest number of fragments and the best sequence coverage compared to pepsin or protease type XVIII from Rhizhopus. Furthermore, protease type XIII exhibited much less self-digestion than pepsin and thus is superior for HDX experiments. Many highly overlapped segments from protease type XIII and pepsin digestion, combined with high-resolution FTICR mass spectrometry, provide high sequence resolution (to as few as one or two amino acids) for the assignment of amide hydrogen exchange rate. Our H/D exchange results correlate well with the secondary and tertiary structure of myoglobin. Such assignments of highly overlapped fragments promise to greatly enhance the accuracy and sequence resolution for determining conformational differences resulting from ligand binding or protein− protein interactions.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekti:
098-0982915-2945 - Spektroskopija, kemijska svojstva i reakcije biološki aktivnih molekula (Kovač, Branka, MZOS ) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Saša Kazazić (autor)

Poveznice na cjeloviti tekst rada:

doi pubs.acs.org pubs.acs.org doi.org

Citiraj ovu publikaciju:

Zhang, Huimin; Kazazić, Saša; Schaub, Tanner; Tipton, Jeremiah; Emmett, Mark; Marshall, Alan
Enhanced Digestion Efficiency, Peptide Ionization Efficiency, and Sequence Resolution for Protein Hydrogen/Deuterium Exchange Monitored by FT-ICR Mass Spectrometry // Analytical chemistry, 80 (2008), 23; 9034-9041 doi:10.1021/ac801417d (međunarodna recenzija, članak, znanstveni)
Zhang, H., Kazazić, S., Schaub, T., Tipton, J., Emmett, M. & Marshall, A. (2008) Enhanced Digestion Efficiency, Peptide Ionization Efficiency, and Sequence Resolution for Protein Hydrogen/Deuterium Exchange Monitored by FT-ICR Mass Spectrometry. Analytical chemistry, 80 (23), 9034-9041 doi:10.1021/ac801417d.
@article{article, author = {Zhang, Huimin and Kazazi\'{c}, Sa\v{s}a and Schaub, Tanner and Tipton, Jeremiah and Emmett, Mark and Marshall, Alan}, year = {2008}, pages = {9034-9041}, DOI = {10.1021/ac801417d}, keywords = {Enhanced Digestion Efficiency, Peptide Ionization Efficiency, }, journal = {Analytical chemistry}, doi = {10.1021/ac801417d}, volume = {80}, number = {23}, issn = {0003-2700}, title = {Enhanced Digestion Efficiency, Peptide Ionization Efficiency, and Sequence Resolution for Protein Hydrogen/Deuterium Exchange Monitored by FT-ICR Mass Spectrometry}, keyword = {Enhanced Digestion Efficiency, Peptide Ionization Efficiency, } }
@article{article, author = {Zhang, Huimin and Kazazi\'{c}, Sa\v{s}a and Schaub, Tanner and Tipton, Jeremiah and Emmett, Mark and Marshall, Alan}, year = {2008}, pages = {9034-9041}, DOI = {10.1021/ac801417d}, keywords = {Enhanced Digestion Efficiency, Peptide Ionization Efficiency, }, journal = {Analytical chemistry}, doi = {10.1021/ac801417d}, volume = {80}, number = {23}, issn = {0003-2700}, title = {Enhanced Digestion Efficiency, Peptide Ionization Efficiency, and Sequence Resolution for Protein Hydrogen/Deuterium Exchange Monitored by FT-ICR Mass Spectrometry}, keyword = {Enhanced Digestion Efficiency, Peptide Ionization Efficiency, } }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





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