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Resolution and peak asymmetry modelling in gradient elution ion chromatography

The separation is usually, globally optimized by taking into account exclusively the retention times of solutes which enables a fast and simple calculation. However, this is suitable for modeling chromatograms that include symmetrical peaks and show similar plate count. Inclusion of the peak size and shape in the optimization process might be of interest when asymmetric or low-efficiency peaks are involved, or when poorly separated symmetrical peaks are observed. There is no universal theoretical model for the exact description of the shape of chromatographic peaks. The aim of this work is development of gradient elution retention model that can be applied for modelling of resolution and peak asymmetry in ion chromatography. Developed gradient elution retention model is based on isocratic experimental data enabling fast and simple modelling of various gradient profiles. This study shows extensive error analysis of developed gradient elution model in order to verify its performance characteristics.

ion chromatography; resolution; modelling; gradient elution

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