Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 367101

Environmental genotoxicity assessment using freshwater crayfish Astacus leptodactylus


Malev, Olga; Šrut, Maja; Maguire, Ivana; Štambuk, Anamaria; Lorenzon, Simonetta; Ferrero, Enrico A.; Klobučar, Göran I.V.
Environmental genotoxicity assessment using freshwater crayfish Astacus leptodactylus // 38th Annual Meeting of European Environmental Mutagen Society (EEMS) : book of abstracts / Franekić-Čolić, Jasna ; Garaj-Vrhovac, Vera (ur.).
Zagreb: Hrvatsko genetičko društvo, 2008. str. 225-225 (poster, domaća recenzija, sažetak, znanstveni)


CROSBI ID: 367101 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Environmental genotoxicity assessment using freshwater crayfish Astacus leptodactylus

Autori
Malev, Olga ; Šrut, Maja ; Maguire, Ivana ; Štambuk, Anamaria ; Lorenzon, Simonetta ; Ferrero, Enrico A. ; Klobučar, Göran I.V.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
38th Annual Meeting of European Environmental Mutagen Society (EEMS) : book of abstracts / Franekić-Čolić, Jasna ; Garaj-Vrhovac, Vera - Zagreb : Hrvatsko genetičko društvo, 2008, 225-225

Skup
Annual Meeting of European Environmental Mutagen Society (38 ; 2008)

Mjesto i datum
Cavtat, Hrvatska, 21.-25.09.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
genotoxicity assessment; comet assay; freshwater crayfish

Sažetak
Genotoxic effects are often one of the earliest signs of pollution-related environmental disturbance. In this study comet assay was applied to assess DNA damage in haemocytes of freshwater narrow-clawed crayfish Astacus leptodactylus exposed to environmental pollution in situ. Animals collected at the reference site (the Mrežnica River) were exposed in cages for one week at three sites of different pollution intensity along the Sava River (Zagreb, Sisak, Krapje). A separate crayfish group caged at the reference site was used as a control. Significant increase in DNA damage measured by the comet assay was observed at all three polluted sites comparing to the reference site. On the polluted site Zagreb, native individuals from the mildly polluted site (Krapje) were cage exposed along with the ones from the reference site. Induced DNA damage was lower in crayfish originating from the Krapje site, indicating higher activity of adaptive mechanisms in animals from pollution-stressed environment. To determine influence of various abiotic factors on crayfish DNA integrity, animals were exposed in laboratory to air, different water temperatures, or were deprived of food. Increased temperature (25°C, 30°C) induced significant increase in DNA damage, but returned to control levels within one week of recovery. Air exposure caused inducement of DNA damage after 6 hours but decreased to control levels within next 18 hours. Two weeks of food deprivation did not cause any detectable genotoxic effects. In this study freshwater crayfish were used for the first time as sentinel organisms in environmental genotoxicity studies. Our results indicate that crayfish are easily adapted to abiotic environmental changes, what is considered preferential for sentinel species. Moreover, we have proved applicability of caging exposure of crayfish A. leptodactylus in freshwater biomonitoring and confirmed usefulness of comet assay as suitable tool for detection of pollution-related genotoxicity.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
119-0000000-3167 - Filogenetske i filogeografske značajke porodice Astacidae u Hrvatskoj
119-0982934-3110 - UČINAK ONEČIŠĆENJA NA GENETIČKU STRUKTURU ORGANIZAMA U VODENOM OKOLIŠU (Klobučar, Goran, MZOS ) ( POIROT)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Anamaria Štambuk (autor)

Avatar Url Olga Malev (autor)

Avatar Url Maja Šrut (autor)

Avatar Url Ivana Maguire (autor)

Avatar Url Goran Klobučar (autor)


Citiraj ovu publikaciju:

Malev, Olga; Šrut, Maja; Maguire, Ivana; Štambuk, Anamaria; Lorenzon, Simonetta; Ferrero, Enrico A.; Klobučar, Göran I.V.
Environmental genotoxicity assessment using freshwater crayfish Astacus leptodactylus // 38th Annual Meeting of European Environmental Mutagen Society (EEMS) : book of abstracts / Franekić-Čolić, Jasna ; Garaj-Vrhovac, Vera (ur.).
Zagreb: Hrvatsko genetičko društvo, 2008. str. 225-225 (poster, domaća recenzija, sažetak, znanstveni)
Malev, O., Šrut, M., Maguire, I., Štambuk, A., Lorenzon, S., Ferrero, E. & Klobučar, G. (2008) Environmental genotoxicity assessment using freshwater crayfish Astacus leptodactylus. U: Franekić-Čolić, J. & Garaj-Vrhovac, V. (ur.)38th Annual Meeting of European Environmental Mutagen Society (EEMS) : book of abstracts.
@article{article, author = {Malev, Olga and \v{S}rut, Maja and Maguire, Ivana and \v{S}tambuk, Anamaria and Lorenzon, Simonetta and Ferrero, Enrico A. and Klobu\v{c}ar, G\"{o}ran I.V.}, year = {2008}, pages = {225-225}, keywords = {genotoxicity assessment, comet assay, freshwater crayfish}, title = {Environmental genotoxicity assessment using freshwater crayfish Astacus leptodactylus}, keyword = {genotoxicity assessment, comet assay, freshwater crayfish}, publisher = {Hrvatsko geneti\v{c}ko dru\v{s}tvo}, publisherplace = {Cavtat, Hrvatska} }
@article{article, author = {Malev, Olga and \v{S}rut, Maja and Maguire, Ivana and \v{S}tambuk, Anamaria and Lorenzon, Simonetta and Ferrero, Enrico A. and Klobu\v{c}ar, G\"{o}ran I.V.}, year = {2008}, pages = {225-225}, keywords = {genotoxicity assessment, comet assay, freshwater crayfish}, title = {Environmental genotoxicity assessment using freshwater crayfish Astacus leptodactylus}, keyword = {genotoxicity assessment, comet assay, freshwater crayfish}, publisher = {Hrvatsko geneti\v{c}ko dru\v{s}tvo}, publisherplace = {Cavtat, Hrvatska} }




Contrast
Increase Font
Decrease Font
Dyslexic Font