engleski

Histopathological evaluation of a dose-responsive tissue reaction associated with wow-formulated vaccine in chicken

This study investigates the tissue responses of a novel double-phase vaccinal emulsion formulated as water-in-oil-in-water (WOW). A total of 120 three-week-old White Leghorn Chickens, hatched from SPF eggs, were randomly assigned to four groups of 30 birds and monitored for the next 4 weeks. The chickens were vaccinated intramuscularly with 0.2, 0.5 and 1.0 ml of oil-based vaccine formulated as WOW emulsion (Pestikal, VETERINA, INC, Croatia) containing inactivated NDV. The control group was injected with 0.5 ml of sterile saline. Injection site tissue reaction was evaluated at 2 and 7-day intervals post inoculation. The severity of histopathological lesions was evaluated using a 1-4 scoring scale. Histopathological lesons in the pectoral muscles were correlated with the dose of injection. Predominant findings, regardless of the inoculated dose, were composed of macrophages, epitheloid cells and small interstitial cysts surrounded by the proliferating fibroblasts, lymphocytes and plasma cells. Lesions, characterized as oleogranuloma, were present in high dose WOW-inoculated chickens and persisted througout the 4 weeks of study. The manufacturer dose, based on the immunological response, is 0.5 ml. Our findings supported a dose of 0.5 ml of the WOW-formulated vaccinal emulsion as optimal in stimulating a local reaction needed for further humoral response.

histopathology; chicken; wow-formulated vaccine; Newcastle Disease virus

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