engleski

Multimodal cell analysis of chronic lymphoproliferative disorders

Introduction: The clinical and morphological picture of the leukemic types of chronic lymphoproliferative disorders (CLLPD) shows considerable heterogeneity. The aim of the study was to correlate the morphometry, nucleolar organization region (AgNOR) and image DNA cytometry (multimodal cell analysis) between lymphatic cells in different tumor mass compartments: bone marrow (BM), peripheral blood (PB) and lymph node (LN). Methods: A total of 657 smears of BM, PB and LN were analyzed by multimodal cell analysis performed on a SFORM PC (VAMSTEC, Zagreb). Statistical analyses were used on Statistica 7.1. Results: Multimodal cell analysis revealed the low proliferative cells to possess small, homogeneous AgNOR with the majority of cells in the peak of DNA histogram. The high proliferative cells had inhomogeneous AgNOR mostly containing greater DNA amount than peak cells or DNA >4N, or the majority of cells being in the S- phase of the cell cycle. Analysis of cell size and proliferative activity in different compartments of tumor mass revealed a regular pattern within total CLLPD population, and typical B-chronic lymphocytic leukemia. Whereas the cells in BM and PB did not differ substantially according to size and proliferative activity, an inverse pattern was observed between PB and LN. As small cells are inactive and larger cells more proliferative the analysis quite unexpectedly showed the PB cells to be largest and most inactive, in contrast to LN where the cells were smallest and most active. Conclusion: Multimodal cell analysis of CLLPD analyzed simultaneously in different tumor mass compartments, provide better assessment of the disease.

Multimodal cell analysis; morphometry; nucleolar organization region (AgNOR); image DNA cytometry; chronic leukemic lymphoproliferative disorders

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