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Glutathione S-transferase P1 polymorphisms in cerebral palsy


Mlinac, Kristina; Vasung, Martina; Grubešić, Zdravko; Žuntar, Irena; Kalanj Bognar, Svjetlana
Glutathione S-transferase P1 polymorphisms in cerebral palsy // Book of Abstracts of the HDBMB 2008, Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation / Strelec, Ivica ; Glavaš-Obrovac, Ljubica (ur.).
Osijek: Croatian Society of Biochemistry and Molecular Biology, 2008. str. 110-110 (poster, domaća recenzija, sažetak, znanstveni)


Naslov
Glutathione S-transferase P1 polymorphisms in cerebral palsy

Autori
Mlinac, Kristina ; Vasung, Martina ; Grubešić, Zdravko ; Žuntar, Irena ; Kalanj Bognar, Svjetlana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the HDBMB 2008, Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation / Strelec, Ivica ; Glavaš-Obrovac, Ljubica - Osijek : Croatian Society of Biochemistry and Molecular Biology, 2008, 110-110

ISBN
978-953-95551-2-0

Skup
HDBMB 2008 Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation

Mjesto i datum
Osijek, Hrvatska, 17-20.09.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Cerebral palsy; glutathione S-transferase P1; C341T polymorphism

Sažetak
Cerebral palsy (CP) is a non-progressive neurodevelopmental disorder of complex ethiopathogenesis, caused by damage to the developing brain which occurs prenatally, perinatally or postnatally. Glutathione S-transferases (GSTs) are a multiple-gene family of enzymes that participate in phase II drug biotransformation, steroidogenesis, cell signalling and detoxification. It comprises of several classes: Alpha (GSTA), Mu (GSTM), Pi (GSTP) and Theta (GSTT). Abnormal antioxidant and detoxification mechanisms may result in cell injury due to the inability to inactivate free radicals and therefore could affect neurodevelopmental processes. GSTP1 is the most important large group of extrahepatical enzymes thought to play a role in detoxification and in susceptibility to some diseases. The aim of this study was to estimate the frequency of A313G (Ile→ Val) and C341T (Ala→ Val) GSTP1 polymorphisms in children with cerebral palsy in comparison with healthy individuals using PCR-RFLP. Genomic DNA was extracted from leukocytes, amplified using specific primers and digested with adequate restriction enzymes. DNA samples from 60 children with CP were investigated and compared with control group of 231 healthy subjects. Obtained results show that there is no significant difference for A313G polymorphism in the frequency of mutated alelle (G) in CP (26.7 %) and healthy subjects (28.6 % ). However, in the case of C341T polymorphism, there is a statistically significant difference in the frequency of the mutated allele (T) between CP (5.83 %) and healthy subjects (27.7 %). Also, in CP subjects we didn't find any homozygotes (TT) while the frequency of TT genotype in healthy subjects is 7.8 %. Statistically significant difference between the healthy control subjects and CP subjects indicates the possible protective role of C341T polymorphism in healthy individuals.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
006-0061245-0010 - Glutation S-transferaza i superoksid dizmutaza u etiopatogenezi bolesti (Irena Žuntar, )
108-1081870-1877 - Uloga membranskih lipida u moždanom razvitku, starenju i neurodegeneraciji (Svjetlana Kalanj-Bognar, )

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
Medicinski fakultet, Zagreb