engleski

Comparison of Bovine Oocyte Recovery and in vitro Embryo Development after Ovum Pick up in Simmental, Charolais and Holstein Friesian Cows

The aim of the present study was to investigate the effect of three cattle breeds on number of aspirated follicles, retrieved oocytes, oocyte recovery rate, oocyte quality and subsequent embryo development after transvaginal Ovum Pick-Up. Transvaginal ultrasound guided (Pie Medical, the Netherlands) oocyte collection was performed in 12 nonlactating donor cows (4 Simmental, 4 Charolais and 4 Holstein-Friesian). Cows were synchronized with PGF2&#61537; and stimulated with pFSH, twice a day during two days (Folltropin&#174; ; , Bioniche, a total dose: 200 mg NIH-FSH-P1). OPU was performed 24 hours after the last FSH injection and procedure was repeated every second week for eight consecutive weeks. The number of aspirated follicles, the number of retrieved oocytes and oocyte recovery rate were recorded. The recovered oocytes were morphologically classified into four (1-4) quality categories. Grade 1 and 2 oocytes were matured, fertilized and cultured in vitro. The culture medium was composed of synthetic oviductal fluid (SOF) medium with aminoacids and 8 mg/mL BSA, and the embryos were cultured for 9 days at 38.5°C in 5% CO2, 5% O2, 90% N2 atmosphere with maximum humidity. The cleavage rates on Day 2, the total number of morulas (M) and blastocysts (Bl) on Day 7 and the numbers of hatched blastocysts (hBl) on Day 9 were recorded. All results were analyzed with ANOVA (StatSoft, Statistic, version 7.1.) and with Tukey&#8217; s tests post-hoc analysis. The OPU results are presented in Table 1. Table 1. Number of aspirated follicles, oocytes recovered and oocyte morphology in Simmental, Charolais and Holstein-Friesian cows (mean &#177; ; S.E.M.) Animal breed Number of follicles punctuired per animal Number of recovered oocytes per animal Oocyte recovery rate per animal Number of grade 1-2 oocytes per animal Number of grade 3-4 oocytes per animal Simmental 14.1&#177; ; 1.1a 9.7&#177; ; 1.1a 68.8&#177; ; 0.08 6.2&#177; ; 0.8 a 1.4&#177; ; 0.3 Charolais 13.1&#177; ; 1.4a 9.1&#177; ; 1.4 a 69.4&#177; ; 0.05 6. 9&#177; ; 1.2 a 1.6&#177; ; 0.2 Holstein-Friesian 8.6&#177; ; 0.5b 4.9&#177; ; 0.5 b 56.9&#177; ; 0.09 3.5&#177; ; 0.5 b 1.2&#177; ; 0.2 abValues with different superscripts differ between columns ((P<0.05) There was no differences in the proportion of good quality (grade 1-2) oocytes undergoing in vitro fertilization and subsequent development to the blastocyst stage between Simmental, Charolais and Holstein-Friesian breed. In conclusion, we reported that Simmental and Charolais donor cows showed significantly better results in number of follicles punctured, total number of oocytes and number of grade 1 and 2 oocytes recovered by OPU, but there was no differences in developmental competence of retreived oocytes of similar quality between three donor breeds.

cow; breed; OPU

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