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Lyme borreliosis infection in fat dormice (Glis glis) determined by PCR-RFLP


Turk, Nenad; Milas, Zoran; Margaletić, Josip; Grubešić, Marijan; Turk, Romana; Barbić, Ljubo; Štritof, Zrinka; Habuš, Josipa; Starešina, Vilim
Lyme borreliosis infection in fat dormice (Glis glis) determined by PCR-RFLP // Abstract Book of the XIIth International Congress of Bacteriology and Applied Microbiology / Dal, Adem (ur.).
Istanbul: Turkish Microciological Society, 2008. str. 316-316 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Lyme borreliosis infection in fat dormice (Glis glis) determined by PCR-RFLP

Autori
Turk, Nenad ; Milas, Zoran ; Margaletić, Josip ; Grubešić, Marijan ; Turk, Romana ; Barbić, Ljubo ; Štritof, Zrinka ; Habuš, Josipa ; Starešina, Vilim

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book of the XIIth International Congress of Bacteriology and Applied Microbiology / Dal, Adem - Istanbul : Turkish Microciological Society, 2008, 316-316

ISBN
0-522-44553-8

Skup
International Congress of Bacteriology and Applied Microbiology (12 ; 2008)

Mjesto i datum
Istanbul, Turska, 05-09.08.2008.

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Lyme borreliosis; fat dormice; PCR-RFLP; Croatia

Sažetak
To determine whether some of the B. burgdorferi sensu lato genospecies associate with fat dormouse as a reservoir host, we investigated the prevalence of infection in questing animals. A total of 45 adult fat dormice (30 female and 15 male) were captured by hunters during their hunting season in the region of Gorski Kotar, Croatia. Dead animals were aseptically dissected and the urinary bladder tissue was used for isolation attempt and for the DNA extraction. Out of 45 DNA samlpes extracted from urinary bladder tissue we found 4 (8.88%) to be PCR positive. The RFLP analysis of the PCR product after cleavage with Dra and Msel distinguished between the three major genospecies: B. burgdorferi sensu stricto, B. garinii and B. afzelii. All positive samples were typed as B. afzelii with a unique Dra or Msel pattern. The results of the analysis of urinary bladder tissue samples culture for the presence of Borelia were negative. Results showed a prevalence of the Borrelia infection among population of fat dormice indicated their epizootiological involvement as a reservoir of Borrelia spirochetes. Furthemore, this work is an initial step in the investigation of the molecular epidemiology/epizootiology of Lyme borreliosis in Croatia.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina, Šumarstvo



POVEZANOST RADA


Projekt / tema
053-0481153-1129 - Mikoplazmoze i neke uvjetovane infekcijske bolesti životinja (Branka Šeol, )
053-1430115-2116 - Molekularna epizootiologija i epidemiologija leptospiroze (Nenad Turk, )
068-0532400-0712 - Utjecaj staništa na prirast i tjelesni razvoj divljači (Marijan Grubešić, )
068-1430115-2119 - Šumski ekosustavi kao prirodna žarišta hantavirusa i leptospira (Josip Margaletić, )

Ustanove
Veterinarski fakultet, Zagreb,
Šumarski fakultet, Zagreb