engleski

Partial Oxygen and Carbon Dioxide Pressure of Exhaled Breath Condensate

Objective: To assess (a) the effect of ambient and exhaled gases on water gas analysis, and (b) the impact of argonization on the results of oxygen (pO2) and carbon dioxide (pCO2) determination in exhaled breath condensate. Methods: Samples of deionized water exposed to atmosphere and exhaled air were analyzed to assess the effect of ambient gases on the respective analyte results. EBC samples were argonized by two methods. Also, pO2 and pCO2 were determined in EBC obtained from healthy children. Results: Deionized water samples exposed to air showed similar pO2 and pCO2 values in ambient air. The values of pO2 were higher, and those of pCO2 lower in water after exhaled air bubbling. The values of pO2 and pCO2 were significantly higher in EBC samples protected by argon layer. Conclusion: Ambient/exhaled gases influence gases analysis in water and EBC, respectively. Additional validation of the method is needed.

exhaled breath condensate; gasses - oxygen; carbon dioxide; O2; CO2

Acknowledgments. The results presented were obtained in the scope of a scientific project No. 277-2770966-0965, entitled Exhaled breath condensate as a source of lung disease biomarkers in children, carried out with support from the Ministry of Science, Education and Sports of the Republic of Croatia.