engleski

SUPEROVULATION RESPONSE AND EMBRYO RECOVERY OF DONOR DOES BOER RACE IN EMBRYOTRANSFER PROCEDURES

This article examines the technique of in vivo production of goat embryos, superovulatory response, embryo recovery of donor does as well as the obstacles associated with those procedures (early luteolysis, anti pFSH antibodies, growths). For that reason does are treated by inserting the vaginal sponges for 11 days and a pFSH treatement for three days in decreasing doses, beggining on the 9th day. 24 hours after removing the sponges, does are hand-mated. 7 day later, medioventral laparotomy is performed and the basis and tip of the horn are punctured. The lavage of each horn is performed by retrograde perfusion. Our research was made on 3 boer race goats. Goats responded with 16 ovulations on average. One of three goats has a early luteolysed CL, and even though the sponge is applied 3 days after mating, the phenomenon wasn't minimized. The lavage of uterus resulted with 4 retarded morulas and 15 unfertilized oocytes. The other 2 goats had 10 and 17 CL respectively, from which 24 embryos are obtained (13 morulas, 3 early blastocysts, 2 blastocysts, 5 expanded and 1 hatched blastocyst). This technique along with the freezing and transfering of obtained embryos should contribute to the genetic improvement of the flocks and should encourage further studies.

superovulation; embryo; doe; transfer

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