Napredna pretraga

Pregled bibliografske jedinice broj: 361762

Influence of interleukin-8 and interleukin-10 on sporadic colon cancer development and progression


Čačev, Tamara; Radošević, Senka; Križanac, Šimun; Kapitanović, Sanja
Influence of interleukin-8 and interleukin-10 on sporadic colon cancer development and progression // Carcinogenesis, 29 (2008), 8; 1572-1580 doi:10.1093/carcin/bgn164 (međunarodna recenzija, članak, znanstveni)


Naslov
Influence of interleukin-8 and interleukin-10 on sporadic colon cancer development and progression

Autori
Čačev, Tamara ; Radošević, Senka ; Križanac, Šimun ; Kapitanović, Sanja

Izvornik
Carcinogenesis (0143-3334) 29 (2008), 8; 1572-1580

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Interleukin-8 ; interleukin-10 ; polymorphism ; mRNA expression ; immunohistochemistry ; colon cancer

Sažetak
Cytokines produced in the tumour microenvironment have an important role in cancer pathogenesis. Altered cytokine expression may result in increased susceptibility to and/or poor prognosis in certain cancers. Therefore the aim of this study was to investigate the influence of interleukin-8 and interleukin-10 on sporadic colon cancer development and progression. In our study, a statistically significant increase in IL-8 mRNA expression and decrease in IL-10 mRNA expression in tumour tissue compared to normal mucous tissue was observed (p=0.003 ; p=1.3x10-9. No association was found between IL-8 -251A/T genotypes and IL-8 mRNA expression in tumour and corresponding normal mucous tissue, as well as susceptibility to sporadic colon cancer. Positive immunohistochemical IL-8 staining was more frequent in moderately and poorly differentiated tumours compared to well differentiated tumours (p=0.024). Finally, interleukin-8 significantly stimulated invasion of HT-29 cells in vitro (p= 0.000172). Significant association of IL-10 -1082 A/G, -819 T/C and -592 A/C genotypes and IL-10 mRNA expression in tumour tissue was observed (p=0.022 ; p=0.013 ; p=0.02). Significant association of -819 T/C and -592 A/C genotypes and IL-10 mRNA expression in corresponding normal mucous tissue was observed (p=0.01 ; p=0.04), as well. IL-10 SNP promoter genotypes associated with low IL-10 mRNA expression (-819 TT ; -592 AA) were also associated with increased risk of sporadic colon cancer compared to high expression genotypes (5.53, 95% CI, 1.53-20.1 ; 4.07, 95% CI, 1.28-12.96). Positive IL-10 immunohistochemical reaction was more frequent in well- and moderately differentiated tumours compared to poorly differentiated tumours (p=0.036). In Dukes’ C tumours positive IL-10 immunohistochemical reaction was less frequent compared to Dukes’ A and B tumours (p=0.023). Taken together our results point to possible tumour promoting role of interleukin-8 and potential protective role of interleukin-10 in sporadic colon cancer.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
098-0982464-2508 - Molekularna genetika i farmakogenetika gastrointestinalnih tumora (Sanja Kapitanović, )

Ustanove
Pliva-Istraživački institut,
Institut "Ruđer Bošković", Zagreb,
Klinička bolnica "Dubrava"

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati