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Purification and properties of extracellular lipase from Streptomyces rimosus


Abramić, Marija; Leščić, Ivana; Korica, Tamara; Vitale, Ljubinka; Saenger, Wolfram; Pigac, Jasenka
Purification and properties of extracellular lipase from Streptomyces rimosus // Enzyme and Microbial Technology, 25 (1999), 6; 522-529 (međunarodna recenzija, članak, znanstveni)


Naslov
Purification and properties of extracellular lipase from Streptomyces rimosus

Autori
Abramić, Marija ; Leščić, Ivana ; Korica, Tamara ; Vitale, Ljubinka ; Saenger, Wolfram ; Pigac, Jasenka

Izvornik
Enzyme and Microbial Technology (0141-0229) 25 (1999), 6; 522-529

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Streptomyces rimosus; bacterial; extracellular; lipase

Sažetak
An extracellular lipase of Streptomyces rimosus R6-554W was isolated from culture filtrate by column chromatography using diethylaminoethyl-cellulose, carboxymethyl-cellulose, hydroxylapatite, Mono S (fast protein liquid chromatography), and Sephadex G-75. It was shown to be a monomeric, basic protein (Mr=27500, pI=8.45), active toward triolein and p-nitrophenyl esters, with preferrence for those with medium size (C8-C12) acyl chain length. Interfacial activation was observed with p-nitophenyl butyrate as substrate. The lipase was most active at 50-60°C and in alkaline conditions around pH 9-10, with p-nitrophenyl palmitate as substrate. It showed high stability at a broad pH range of 4-10 and was fairly thermostable. Dithiothreitol moderately inactivated the enzyme. Phenylmethylsulfonyl fluoride partly inhibited lipase only when added during the hydrolytic reaction.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
00980608
00980705

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus