engleski

Copper determination by ETAAS in fish tissue cytosols with minimal sample pretreatment

The metal concentrations in the fish tissue cytosols offer an important parameter in environmental monitoring studies as they reflect metal bioavailability in ambient water. The aim of this study was to examine the possibility of direct Cu determination in the cytosols of chub gills and intestines by electrothermal atomic absorption spectrometry (ETAAS) with minimal sample pretreatment and without the use of modifiers. Tube wall and platform atomizations were tested. When tube wall atomization was applied, a progressive decline of Cu absorbance was observed with increasing firing count. On the other hand, a stability of Cu absorbance throughout the graphite tube lifetime (up to 250 firings) was obtained with platform atomization. Signal stability, together with excellent measurement repeatability ( 5%) and acceptable Cu recovery from spiked samples (77-89%), make ETAAS with platform atomization applicable to the determination of low Cu concentrations (<27  g L-1) in environmental samples with complex organic matrices, such as undigested fish tissue cytosols, even without the use of chemical modifiers.

copper; cytosol; organic matrix; ETAAS; platform atomization; tube wall atomization

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