Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 351003

Reaction of Trigonopsis variabilis D-amino acid oxidase with 2, 6-dichloroindophenol: kinetic characterization and development of an oxygen-independent assay of the enzyme activity


Trampitsch, Christian; Slavica, Anita; Riethorst, Waander; Nidetzky, Bernd
Reaction of Trigonopsis variabilis D-amino acid oxidase with 2, 6-dichloroindophenol: kinetic characterization and development of an oxygen-independent assay of the enzyme activity // Journal of Molecular Catalysis B - Enzymatic, 32 (2005), 5-6; 271-278 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 351003 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Reaction of Trigonopsis variabilis D-amino acid oxidase with 2, 6-dichloroindophenol: kinetic characterization and development of an oxygen-independent assay of the enzyme activity

Autori
Trampitsch, Christian ; Slavica, Anita ; Riethorst, Waander ; Nidetzky, Bernd

Izvornik
Journal of Molecular Catalysis B - Enzymatic (1381-1177) 32 (2005), 5-6; 271-278

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
flavoenzyme; electron acceptor; oxygen; biocatalysis; enzyme assay

Sažetak
2, 6-Dichloroindophenol (DCIP) is shown to be utilised efficientlz as electron acceptor replacing dioxygen in the reaction of Trigonopsis variabilis D-amino acid oxidase (TvDAO) with D-Methionine as the susbstrate. The specifificty constant for DCIP reduction at 30 C is one twelfth that of oxygen conversion into hydrogen peroxide. Time course analysis of symultaneous consumption of DCIP and dioxygen, recorded on-line by absorption and non-invasive fluorescence quenching, respectively, pinpoints the preferential utilisation of dioxygen ; and reveals a maximum DCIP conversion rate that is independent of the initial concentration of dioxygen. A robust direct assay of TvDAO activity has been developed that does not require ananerobic reaction conditions. It was down-scaled to microtitre plate format and overcomes practical limitations of other assays due to low affnity of TvDAO for dioxygen (Km  0.7 mmol L-1).

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekti:
0058011

Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb

Profili:

Avatar Url Anita Slavica (autor)


Citiraj ovu publikaciju

Trampitsch, Christian; Slavica, Anita; Riethorst, Waander; Nidetzky, Bernd
Reaction of Trigonopsis variabilis D-amino acid oxidase with 2, 6-dichloroindophenol: kinetic characterization and development of an oxygen-independent assay of the enzyme activity // Journal of Molecular Catalysis B - Enzymatic, 32 (2005), 5-6; 271-278 (međunarodna recenzija, članak, znanstveni)
Trampitsch, C., Slavica, A., Riethorst, W. & Nidetzky, B. (2005) Reaction of Trigonopsis variabilis D-amino acid oxidase with 2, 6-dichloroindophenol: kinetic characterization and development of an oxygen-independent assay of the enzyme activity. Journal of Molecular Catalysis B - Enzymatic, 32 (5-6), 271-278.
@article{article, year = {2005}, pages = {271-278}, keywords = {flavoenzyme, electron acceptor, oxygen, biocatalysis, enzyme assay}, journal = {Journal of Molecular Catalysis B - Enzymatic}, volume = {32}, number = {5-6}, issn = {1381-1177}, title = {Reaction of Trigonopsis variabilis D-amino acid oxidase with 2, 6-dichloroindophenol: kinetic characterization and development of an oxygen-independent assay of the enzyme activity}, keyword = {flavoenzyme, electron acceptor, oxygen, biocatalysis, enzyme assay} }
@article{article, year = {2005}, pages = {271-278}, keywords = {flavoenzyme, electron acceptor, oxygen, biocatalysis, enzyme assay}, journal = {Journal of Molecular Catalysis B - Enzymatic}, volume = {32}, number = {5-6}, issn = {1381-1177}, title = {Reaction of Trigonopsis variabilis D-amino acid oxidase with 2, 6-dichloroindophenol: kinetic characterization and development of an oxygen-independent assay of the enzyme activity}, keyword = {flavoenzyme, electron acceptor, oxygen, biocatalysis, enzyme assay} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus





Contrast
Increase Font
Decrease Font
Dyslexic Font