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Trigonopsis variabilis D-Amino Acid Oxidase:Control of Protein Quality and Opportunities for Biocatalysis through Production in Escherichia coli


Dib, Iskandar; Stanzer, Damir; Nidetzky, Bernd
Trigonopsis variabilis D-Amino Acid Oxidase:Control of Protein Quality and Opportunities for Biocatalysis through Production in Escherichia coli // Applied and Environmental Microbiology, 73 (2007), 1; 331-332 (međunarodna recenzija, članak, znanstveni)


Naslov
Trigonopsis variabilis D-Amino Acid Oxidase:Control of Protein Quality and Opportunities for Biocatalysis through Production in Escherichia coli

Autori
Dib, Iskandar ; Stanzer, Damir ; Nidetzky, Bernd

Izvornik
Applied and Environmental Microbiology (0099-2240) 73 (2007), 1; 331-332

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
D-amino acid oxidase; biocatalysis; microheterogeneity; recombinant production; oriented imobilization

Sažetak
Trigonopsis variabilis d-amino acid oxidase accounts for 35% of Escherichia coli protein when added d-methionine suppresses the toxic activity of the recombinant product. Permeabilized E. coli cells are reusable and stabilized enzyme preparations. The purified oxidase lacks the microheterogeneity of the natural enzyme. Oriented immobilization of a chimeric oxidase maintains 80% of the original activity in microparticle-bound enzymes.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekt / tema
058-0580477-0374 - Proizvodnja biološki aktivnih tvari pomoću selekcioniranih kvasaca (Damir Stanzer, )

Ustanove
Prehrambeno-biotehnološki fakultet, Zagreb

Autor s matičnim brojem:
Damir Stanzer, (237292)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE