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Roles of ExoI and SbcCD nucleases in "reckless" DNA degradation in recA mutants of Escherichia coli


Zahradka, Ksenija; Buljubašić, Maja; Petranović, Mirjana; Zahradka, Davor
Roles of ExoI and SbcCD nucleases in "reckless" DNA degradation in recA mutants of Escherichia coli // Journal of bacteriology, 191 (2009), 5; 1677-1687 doi:10.1128/JB.01877-07 (međunarodna recenzija, članak, znanstveni)


Naslov
Roles of ExoI and SbcCD nucleases in "reckless" DNA degradation in recA mutants of Escherichia coli

Autori
Zahradka, Ksenija ; Buljubašić, Maja ; Petranović, Mirjana ; Zahradka, Davor

Izvornik
Journal of bacteriology (0021-9193) 191 (2009), 5; 1677-1687

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Escherichia coli ; recA mutants ; DNA degradation ; ExoI ; SbcCD

Sažetak
Exponentially growing recA mutant cells of Escherichia coli display pronounced spontaneous DNA degradation that starts at the sites of DNA damage, and depends on the RecBCD nuclease (ExoV) activity. As a consequence of this "reckless" DNA degradation, populations of recA mutants contain a large proportion of anucleate cells. We have found that both DNA degradation and anucleate cell production are efficiently suppressed by mutations in xonA (sbcB) and sbcD genes. The suppressive effect of these mutations was observed in normally grown, as well as in UV-irradiated, recA cells. The products of xonA and sbcD genes are known to code for ExoI and SbcCD nucleases, respectively. Since both xonA and sbcD mutations are required for strong suppression of DNA degradation while individual mutations have only a weak suppressive effect, we infer that ExoI and SbcCD play partially redundant roles in regulating DNA degradation in recA cells. We suggest that their role might be in processing (blunting) DNA ends, thereby producing suitable substrates for RecBCD binding.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
098-0982913-2862 - Molekularni mehanizmi rekombinacije i popravka DNA (Davor Zahradka, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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