engleski

Degradation of aflatoxin B1 by Trichothecium roseum and Fusarium sp.

Biological degradation of aflatoxin B1 by Trichothecium roseum and Fusarium sp. was examined in yeast extract-sucrose (YES) medium under deifferent incubation conditions. Identification of aflatoxins in 1961 as the agents responsible for severe economic losses by the British turkey industry and the subsequent toxicological information, particularly identifying aflatoxin B1 as a potential liver carcinogenic in certain animal species, may be regarded as the fuse that led to an explosion in research efforts directed not only against aflatoxins, but also many other mycotoxins. Besides attempts to eliminate mycotoxins from contaminated substrates by physical nand chemical methods, the ability of microbes to degrade mycotoxins is now being widely examined. In this study, several T. roseum and Fusarium sp. examined for their ability to degrade AFB1. T. roseum ZMPBF 1226 and Fusarium ZMPBF 1244 were found to detoxify AFB1 in culture media. The kinetics of AFB1 detoxification by an atoxigenic T. rosum strain was examined by thin layer chromatography and ELISA method. T. roseum ZMPBF 1226 was found to effectively eliminate AFB1 from both solid and liquid media ( 28 % AFB1 residual after 5 days and only 10-14 % residual AFB 1 qafter 15 days).

mycotoxins; aflatoxins; degradation; T. roseum; Fusarium sp.

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