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Pregled bibliografske jedinice broj: 350045

Selective modification of surface exposed thiol groups in Trigonopsis variabilis D-amino acid oxidase using poly(ethylene glycol) maleimide and its effect on activity and stability of the enzyme


Slavica, Anita; Dib, Iskandar; Nidetzky, Bernd
Selective modification of surface exposed thiol groups in Trigonopsis variabilis D-amino acid oxidase using poly(ethylene glycol) maleimide and its effect on activity and stability of the enzyme // Biotechnology and Bioengineering, 96 (2007), 1; 9-17 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 350045 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Selective modification of surface exposed thiol groups in Trigonopsis variabilis D-amino acid oxidase using poly(ethylene glycol) maleimide and its effect on activity and stability of the enzyme

Autori
Slavica, Anita ; Dib, Iskandar ; Nidetzky, Bernd

Izvornik
Biotechnology and Bioengineering (0006-3592) 96 (2007), 1; 9-17

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
D-amino acid oxidase; PEG-conjugate; (thermo) stabilization; cysteine modification; FAD; cofactor dissociation

Sažetak
Covalent modification of purified Trigonopsis variabilis D-amino acid oxidase using maleimide-activated poly(ethylene glycol) 5000 yielded a stable bioconjugate in which three surface-exposed cysteine side chains were selectively derivatized. Compared with the native enzyme, the PEGylated variant displayed substantially ( 3.3-fold) slowed dissociation rate of FAD cofactor at 50°C, and this caused a twofold thermostabilization of the enzyme activity. The stability under reaction conditions at 30°C was also markedly enhanced in the PEG-oxidase conjugate. PEGylation did not affect steady-state kinetic parameters for oxidative deamination of D-methionine when 2, 6-dichloroindophenol replaced dioxygen as the cosubstrate while it caused a ninefold decrease in substrate catalytic efficiency for the dioxygen-dependent reaction.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekti:
058-0581990-1997 - Primjena integriranih bioprocesa u proizvodnji mliječne kiseline (Novak, Srđan, MZOS ) ( POIROT)

Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb

Profili:

Avatar Url Anita Slavica (autor)

Citiraj ovu publikaciju

Slavica, Anita; Dib, Iskandar; Nidetzky, Bernd
Selective modification of surface exposed thiol groups in Trigonopsis variabilis D-amino acid oxidase using poly(ethylene glycol) maleimide and its effect on activity and stability of the enzyme // Biotechnology and Bioengineering, 96 (2007), 1; 9-17 (međunarodna recenzija, članak, znanstveni)
Slavica, A., Dib, I. & Nidetzky, B. (2007) Selective modification of surface exposed thiol groups in Trigonopsis variabilis D-amino acid oxidase using poly(ethylene glycol) maleimide and its effect on activity and stability of the enzyme. Biotechnology and Bioengineering, 96 (1), 9-17.
@article{article, year = {2007}, pages = {9-17}, keywords = {D-amino acid oxidase, PEG-conjugate, (thermo) stabilization, cysteine modification, FAD, cofactor dissociation}, journal = {Biotechnology and Bioengineering}, volume = {96}, number = {1}, issn = {0006-3592}, title = {Selective modification of surface exposed thiol groups in Trigonopsis variabilis D-amino acid oxidase using poly(ethylene glycol) maleimide and its effect on activity and stability of the enzyme}, keyword = {D-amino acid oxidase, PEG-conjugate, (thermo) stabilization, cysteine modification, FAD, cofactor dissociation} }
@article{article, year = {2007}, pages = {9-17}, keywords = {D-amino acid oxidase, PEG-conjugate, (thermo) stabilization, cysteine modification, FAD, cofactor dissociation}, journal = {Biotechnology and Bioengineering}, volume = {96}, number = {1}, issn = {0006-3592}, title = {Selective modification of surface exposed thiol groups in Trigonopsis variabilis D-amino acid oxidase using poly(ethylene glycol) maleimide and its effect on activity and stability of the enzyme}, keyword = {D-amino acid oxidase, PEG-conjugate, (thermo) stabilization, cysteine modification, FAD, cofactor dissociation} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus





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