engleski

In-vitro activity of dehydroacetic acid and the newly synthesized Schiff base against mycotoxigenic fungus Aspergillus flavus ATCC 26949 growth and aflatoxin accumulation

The potential for inhibition of aflatoxin accumulation by aflatoxigenic fungus Aspergillus flavus ATCC 26949 was investigated using the dehydroacetic acid and the newly synthesized Schiff base in 3-/2- aminopheylimino (p-toluoyl)/-4-hidroxy-6-(p-toplyl)-2H-pyran-2-one in YES medium at pH 5.5. YES medium was treated with various amounts of DHA and Schiff base after inoculation with A. flaus. Experiments were carried out in a stationary culture at temperatures of 20, 25 and 30oC during 42 days. Mycelial dry weights were determined gravimetrically and concentration of aflatoxin B1 and total aflatoxin (AFT) were measured by using ELISA method. DHA concentrations of 0.5 mikromol/L and 1.o mikromol/L, respectively, stimulated mould growth and AFB1 and AFT accumulation, but concentration higher than 10.0 mikromol/L produced an inhibitory effect. In the presence of low Schiff base concentrations, mould growth was decreased by 75 % concentrations of AFB1 and AFT by 65 % or copmpletely.

Dehydroacetic acid (DHA); Schiff base; mycotoxigenic fungus; aflatoxins

nije evidentirano