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Antitumor effectiveness of propolis preparations and combination of different flavonoids on primary culture of human urinary bladder carcinoma

The flavonoids present in propolis are considered to be rich source of chemopreventive agents, since they have various therapeutic biological activities. This study was carried out to find whether propolis preparations or polyphenolic/flavonoids components of it given alone or in various combinations induce cytotoxicity in primary culture of human urinary bladder cancer cells as compared to normal urinary bladder epithelial cells. Pieces of urinary bladder cancer as well as normal epithelial tissue from urinary bladder wall were taken from patients in different stages (grade G1, G2, G3) of bladder carcinoma. Single cells suspension from pieces of either cancer or normal tissue was prepared according standard laboratory procedure. Incubation for cytotoxicity testing were carried out in RPMI-1640 medium with 20% FCS at 37˚ C with 5% CO2 with or without different concentrations (50, 150, 300 &micro ; g/ml) of test components. The cytotoxicity of two preparations of propolis (water and ethanolic extract of propolis ; WSDP or EEP) and its polyphenolic compounds (caffeic acid, naringin, chrysin, and quercetin) was determined using trypan blue exlusion assay. Findings suggest that EEP wasthe most effective in inhibition of urinary bladder cancer cell proliferation as compared to flavonoids derived from propolis. All test components have no cytotoxic effect on normal epithelial cells. The result of this study may provide great impact on the potential activity of EEP as an adjuvant to surgery, to suppress or prevent tumor recurrence in urinary bladder since only a few anti-cancer drugs have been effective in tumor control. Since immunomodulation by BCG has been used to improve the results of surgery it is likely that propolis preparation (EEP) as immunomodulating compound may be a substitute for mycobacterial treatment since propolis preparation have expressed no side effect after treatment.

human urinary bladder carcinoma; primary culture; propolis preparation; flavonoids; cytotoxicity

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